维生素C对低硒低铬饲料饲养大鼠肝细胞电生理的影响

目的阐明微量元素Se、Cr含量异常时肝细胞损害的机理。方法用细胞内微电极技术分别观察了：正常对照(两组)、单纯低Se、低Se高维生素C、单纯低cr、低Cr’高维生素C、低Se低Cr、低Se低Cr伴高维生素C饲料饲养14w后大鼠肝细胞电生理的改变。结果单纯低铬组无变化；单纯低硒组RP、APA降低，APD50、APD50延长；低硒并低铬组RP、APA降低，APD50、APD90明显延长；低Se高维生素C组仅RP降低，APA、APD与对照组相比差异无显著性意义。结论低硒并低铬可明显改变肝细胞的电生理特性，维生素C可以对抗低Se并派Cr所致的这种变化。

Effects of vitamin C on the electrophysiological property of liver cells of the rats fed with low selenium and low chromium fodder

Objective: To investigate the effects of vitamin c on the electrophysiological property of liver cells of the rats fed with low selenium and low chromium fodder. Methods: With intracellular microelectrode technique, we observed the electrophysiological changes of the eight groups of rats fed separately with: normal (2 groups), low Se, low Se+high VC, low Cr, low Cr+high VC, low Se+low Cr and low Se+low Cr+high VC fodder. Results: Compared with the control, there was no change in low Cr group; However, in low Se group, RP and APA decreased, APD_(50) and APD_(90) prolonged; In low Se+low Cr group, APA decreased, APD_(50) and APD_(90) prolonged significantly; In low Se with high vitamin C groups, though RP still decreased, no change has been detected on APA and APD. Conclusion: It is suggested that Se and Cr deficiency may jointly change the electrophysiological properties of liver cells significantly, however, vitamin C may protect cells against the injury effectively.