The isolation of rat lung cells for the purpose of studying drug metabolism |
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| Authors: | Janet R. Dawson Kajsa Norbeck Peter Moldéus |
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| Affiliation: | Department of Forensic Medicine, Karolinska Institutet, Box 60400, S-104 01 Stockholm, Sweden |
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| Abstract: | A method was developed for the isolation of viable cells from rat lungs. The cells were a heterogeneous population, which maintained a high percentage viability for up to 3 hr on incubation at 37°. Reduced cofactor levels (NADH, NADPH) decreased on incubation, but ATP remained constant. The cells were active in the metabolism of the two xenobiotics studied. A model compound, 7-ethoxycoumarin, was O-deethylated and subsequently conjugated with glucuronic acid and sulphate, whilst a pharmaceutical agent, N-acetylcysteine, was de-acetylated. |
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| Keywords: | To whom all correspondence should be addressed. |
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