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| 树脂理化特性对分离葛根总黄酮的影响 | |
| 引用本文： | 方俊, 王建华. 树脂理化特性对分离葛根总黄酮的影响[J]. 中国现代应用药学, 2010, 27(4): 281-284. |
| 作者姓名： | 方俊 王建华 |
| 作者单位： | 1.重庆大学生物工程学院，重庆 400044 |
| 基金项目： | 人事部留学人员基金项目(20071749); 重庆市自然科学基金项目(2006BB5017); 国家科技支撑计划(2007BAD51B05) |
| 摘 要： | 目的 研究吸附树脂理化性质对分离葛根总黄酮的影响。方法 采用分光光度法分别对5种ADS系列吸附树脂作静态、动态研究，并对较优树脂进行动态吸附-脱附工艺参数的优化。结果 静态实验表明，树脂的极性和空间结构是影响吸附效果的重要因素；树脂ADS-8上柱液浓度约为0.453 mg·mL-1，pH 5~6，吸附速率4 BV·h-1，400 mL蒸馏水洗去杂质，洗脱剂为70%乙醇，洗脱流速2 BV·h-1，用量为60 mL。结论 ADS-8是分离纯化葛根总黄酮的较优树脂。 |
| 关 键 词： | ADS吸附树脂 葛根总黄酮 分离纯化 |
Impact of Physical and Chemical Characteristics of Resin on Separating Total Flavones from Radix Puerariae Lobatae |
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| FANG Jun, WANG Jianhua. Impact of Physical and Chemical Characteristics of Resin on Separating Total Flavones from Radix Puerariae Lobatae[J]. Chinese Journal of Modern Applied Pharmacy, 2010, 27(4): 281-284. | |
| Authors: | FANG Jun WANG Jianhua |
| Affiliation: | 1.College of Bioengineering, Chongqing University, Chongqing 400044,China |
| Abstract: | OBJECTIVE Study on the impact of the physical and chemical characteristics of resin on separating total flavones from Radix Puerariae Lobatae.METHODS Five kinds of ADS adsorbing resins were tested in the static and dynamic research about separating and purifying total flavones.And the best resin was screened out,then its technical parameters in dynamic adsorption-desorption were optimized.RESULTS The static adsorption tests showed that the polarity and textual structure were main factors on the adsorptive c... |
| Keywords: | ADS adsorbing resins total flavones from Radix Puerariae Lobatae separation and purification |
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