脑泰方对局灶性脑缺血大鼠血清MMP-9、PA及TIMP-1表达的影响

目的研究脑泰方治疗对局灶性脑缺血大鼠血清中基质金属蛋白酶9（MMP-9）,MMP-9前体纤溶酶原激活物（plasminogen activator,PA）及内源性抑制剂1（TIMP1）的作用。方法随机将SD大鼠分为假手术组、空白对照组、脑泰方低、中、高剂量组（3,9,27g/kg）,marimastat（BB-2516）组（6.25mg/kg）ig。各组大鼠预处理ig给药连续3d,每日1次,后采用大脑中动脉栓塞法（Middle cerebral artery occlusion,MCAO）行大鼠局灶性脑缺血模型制备,术后连续ig给药3d。采用ZeaLonga神经学评分标准记录动物的行为活动,采用ELISA法检测血清中MMP-9、PA及TIMP1的表达。结果脑泰方高剂量组神经学评分较空白对照组有显著降低（P〈0.05）;脑泰方高、中、低剂量组血清中MMP-9、PA的表达显著降低（P〈0.05）,脑泰方高、中、低剂量组TIMP1表达明显升高（P〈0.05）。结论脑泰方对局灶性脑缺血导致的血脑屏障损伤有一定保护作用。

Effects of Naotaifang on Serum MMP-9,PA and TIMP-1 after Focal Cerebral Ischemia in Rats

Objective To observe Effects of NaoTai Fang on serum MMP-9, PA and TIMP-1 after Focal Cerebral Ischemia in Rats. Methods The rats were randomly allotted 5 groups as following .. sham operation group, model group, low dose group of NTF （3g/kg）, medial dose group of NTF （gg/kg）, large dose group of NTF （27g/kg）, marirnastat （BB-2516） group （6.25mg/kg） . After 3 days of corresponding ther- apy by intragastric administration once every day, The regional cerebral ischemia model was reproduced by Middle cerebral artery occlusion （MCAO） with suture method. Following 3 days, the rats treated with previous method. The third day, neuroethology expression was detected by Zea Longa neurological score. The contents of MMP-9, PA and TIMP1 proteins in serum were measured by enzyme-linked immuno sorbent assay （ELISA） method with marketable kit. Results In large dose group of NTF the expression of neurological score was significantly decrease than model group （ P 〈0. 05） ; In low , medial , large dose group of NTF and marimastat （BB-2516） group the expressions Of MMP-9. PA were less significantly decreased than model group （ P 〈0.05）, and expressions Of TIMP1 were significantly increased than model group （ P 〉 0. 05） . Conclusion The mechanism of NTF protecting brood brain barrier （BBB） against injure of cerebral ischemia may be involved in amelio-rating MMP, PA and increasing TIMP1 expression.