VEGF165反义RNA表达对人胃癌细胞恶性生物学行为的影响

目的利用本室构建的反义VEGF165（血管内皮生长因子，vascular endothelial growth factor）真核表达载体，研究VEGF反义RNA表达对人胃癌细胞生物学表型的影响. 方法用阳性脂质体介导的基因转染技术，将VEGF反义RNA重组体转入人胃癌细胞系，观察转染细胞的细胞周期、增殖能力及致瘤生长情况等生物学表现. 结果 VEGF反义RNA重组体转染胃癌细胞后，斑点杂交、间接免疫荧光等分析显示，反义RNA基因转染技术可使VEGF的表达明显减弱；与未转染的细胞相比，G2/M期细胞增加(0.39)，而S期细胞减少(0.54)；克隆形成率(2.2%)低于未转染细胞(4.0%)；肿瘤细胞接种裸鼠后33 d时，VEGF反义RNA表达人胃癌细胞所致的移植瘤体积（345±136) mm3明显小于未转染细胞所致的移植瘤体积（1534±363) mm3. 结论 VEGF反义RNA可以通过抑制肿瘤组织血管生成而防治肿瘤的生长，另外VEGF的表达可能与细胞增殖能力有关.

VEGF165 antisense gene and biological characteristics of human gastric cancer cells

AIM　To investigate the effect of vascular endothelial growth factor (VEGF) antisense gene on the biological characteristics of human gastric cancer cells by antisense nucleotide technique. METHODS　The VEGF165 antisense gene recombinant was introduced into gastric cancer cell line by lipofect transfection technique. Then the cell cycle, proliferative abiliyt in vitro and growth in nude mice of transfected cells were examined. RESULTS　Introduction of the VEGF165 antisense construct into SGC-7901 cells resulted in a marked reduction in the expression of VEGF-specific mRNA and protein by dot blot and immunofluorescence staining analysis in transfected tumor cells. Cell cycle analysis showed that compared with the parental cells, the number of transfected cells had an increase (0.39) in G2/M phase, a decrease (0.54) in S phase, and its colony forming rate (2.2%) was lower than that (4.0%) of the control cells. The volume of tumor （345±136) mm3 in the nude mice inoculated with the cells transfected by antisense VEGF165 gene construct was greatly reduced in comparison with that （1534±363) mm3 in the nude mice inoculated with SGC-7901 cells. CONCLUSION　As repressing angiogesis, antisense VEGF gene transfection might inhibit the growth of solid tumor; in addition, it might correlate with the proliferation of tumor cells.