| 孕妇血清和胚胎组织中微小病毒B19 DNA的检出 |
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| 引用本文： | 孟潞英 张文炳. 孕妇血清和胚胎组织中微小病毒B19 DNA的检出[J]. 解放军医学杂志, 1993, 18(5): 333-335 |
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| 作者姓名： | 孟潞英 张文炳 |
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| 作者单位： | 第一军医大学微生物学教研室 510515(孟潞英，张文炳)，第一军医大学微生物学教研室 510515(彭华国) |
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| 摘 要： | 作者建立了检测人微小病毒Ｂ１９（ＨＰＶ－Ｂ１９）的ＰＣＲ方法。两对引物（Ｐ１Ｐ２和Ｐ３Ｐ４）的设计均位于编码ＨＰＶ－Ｂ１９衣壳蛋白ＶＰ１基因区内，扩增产物分别为７００ ｂｐ和１０４ ｂｐ。比较Ｐ１Ｐ２－ＰＣＲ和Ｐ３Ｐ４－ＰＣＲ，二者均具有高度特异性和第三性，ＨＰＶ－Ｂ１９ ＤＮＡ最小检同量分别为５ ｆｇ。应用Ｐ１Ｐ２－ＰＣＲ对３２份孕妇血清和２１份胚胎组织标本进行了ＨＰＶ－Ｂ１９ ＤＮＡ的检测，结
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| 关 键 词： | 微小病毒B19 聚合酶链反应 DNA |
DETECTION OF HUMAN PARVOVIRUS B_(19) DNA FROM SERA OF PREGNANT AND FETAL TISSUES |
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| Meng Luying,Zhang Wenbing,Peng Huaguo. First Military Medical University of PLA. Guangzhou. DETECTION OF HUMAN PARVOVIRUS B_(19) DNA FROM SERA OF PREGNANT AND FETAL TISSUES[J]. Medical Journal of Chinese People's Liberation Army, 1993, 18(5): 333-335 |
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| Authors: | Meng Luying Zhang Wenbing Peng Huaguo. First Military Medical University of PLA. Guangzhou |
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| Affiliation: | Meng Luying,Zhang Wenbing,Peng Huaguo. First Military Medical University of PLA. Guangzhou 510515 |
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| Abstract: | The PCR assay for detection of human parvovirus B19(HPV-B19) DNA was developed: P,P2-PCR and P3P4-PCR. The amplified products were 700 bp and 104 bp, respectively. They were all located on the gene sequence which encoded capsid protain VP,of HPV B19. The HPV-B19PCR assay had high specificity and sensitivity. The 5 fg and 25 fg of HPV-B19DNA could be detected by P1P2-PCR and P3P4-PCR separately. The HPV B19DNA in 32 sera of pregnant women and 21 fetal tissues were positive for HPV-B19DNA. The positive percentages were 9.4% (3 / 32) and 9.5% (2 / 21) separately. It was first proved that HPV B19was present in China by DNA detection. |
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| Keywords: | Human parvovirus B19(HPV-B19) Polymerase chain reaction (PCR) Sera of pregnant women Fetal tissuess |
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