| HPLC法测定复方苦参乳膏中苦参碱的质量 |
| |
| 引用本文： | 吴杨,吴银生.HPLC法测定复方苦参乳膏中苦参碱的质量[J].抗感染药学,2012,9(1):38-40. |
| |
| 作者姓名： | 吴杨 吴银生 |
| |
| 作者单位： | 1. 苏州惠氏制药有限公司,江苏苏州,215128
2. 江苏省苏州药品检验所,江苏苏州,215002 |
| |
| 摘 要： | 目的:建立复方苦参乳膏中苦参碱的HPLC质量测定方法。方法:采用Phenomenex C18色谱柱(250mm×4.6mm,5μm),流动相为0.02mol/L三乙胺(冰醋酸调pH=9.5)-乙腈(65∶35),检测波长为209nm。结果:苦参碱的线性范围为4.86~194.40μg/mL(r=0.9999),平均回收率为98.91%(RSD=2.78%,n=6),苦参碱的平均质量分数为1.15mg/g。结论:本方法操作快速简便、准确,适用于复方苦参乳膏中苦参碱的质量测定。
|
| 关 键 词： | 复方苦参乳膏 苦参碱 含量测定 |
Determination of Matrine in Compound Kushen Cream by HPLC |
| |
| WU Yang
,
WU Yin-sheng.Determination of Matrine in Compound Kushen Cream by HPLC[J].Anti-infection Pharmacy,2012,9(1):38-40. |
| |
| Authors: | WU Yang WU Yin-sheng |
| |
| Institution: | 1Wyeth Pharmaceutical Co., Ltd, Suzhou Jiangsu 215128, China; 2Jiangsu Suzhou Institute For Drug Control, SuZhou Jiangsu 215002, China |
| |
| Abstract: | Objective: To establish a method for measuring the contents of matrine in compound Kushen cream. Methods: The separation was carried out on Phenomenex C18 column(250 mm×4.6 mm,5 μm). 0.02 mol/L triethylamine(adjusted to pH 9.5 by acetic acid)-acetonitrile(65:35) was used as a mobile phase. The flow rate was 1.0 mL/min. The detect wavelength was set at 209 nm. Results: The linear ranger of matrine was between 4.86 and 194.4 μg/mL (r=0.999 9) and the average recovery was 98.91% (RSD=2.78%, n=6).The average content of matrine was 1.15 mg/g.Conclusion: This method is simple, fast and accurate, and it is suitable for the determination of matrine in compound Kushen cream. |
| |
| Keywords: | compound Kushen cream matrine determination |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
