增殖细胞核抗原在喉上皮癌前病变中的表达及意义

用免疫组化技术对１１例喉上皮单纯性增生（ＳＨＥ），３２例非典型性增生（ＡＨＥ）及４２例喉鳞状细胞癌（ＬＳＣＣ）的增殖细胞核抗原（ＰＣＮＡ）表达进行了原位检测。结果表明；在ＳＨＥ、ＡＨＥ及ＬＳＣＣ中，ＰＣＮＡ指数分别为９．５７％、２７．３３％、６８．０５％，差异极有显著性意义（Ｐ＜０．００１）。在轻、中、重度ＡＨＥ中，ＰＣＮＡ指数分别为１３．７９％、３０．８４％、３９．９４％，差异有非常显著性意义（Ｐ＜０．０１）。提示ＰＣＮＡ表达程度与非典型性增生程度密切相关，是反映细胞增殖状态的生物标志，ＰＣＮＡ组化研究有助于认识喉癌前病变的本质，发展趋势及喉癌的早期诊断。

Expression of Proliferating Cell Nuclear Antigen(PCNA)in Premalignant Lesions of Laryngeal Epithelium

It is important to study the proliferative activity of cells in the premalignant lesions of laryngeal epithelium.Using PC-10,an antibody to PCNA and a standard immunohistochemical stain-ing,we examined 11 cases of simple hyperplasia of epithelium(SHE),32 cases of atypical hyperplasia of epithelium(AHE) and 42 cases of laryngeal squamous cell carcinoma(LSCC) for expression of PCNA,aprotein associated with DNA polymerase delta and DNA replication.The results revealed that the PCNA indices in SHE,AHE and LSCC were 9.57%,27.33% and 68.05%,respectively.The PCNA indices were 13.79% 30.84% and 39. 94%, in the mild,moderate and severe AHE,respectively.There were significant differences among the SHE,AHE and LSCC,A good correlation was found among different degrees of AHE. The pattern and location of PCNA positive cells in the intraepithelium had diagnostic importance.PCNA might provide a useful tool for studying cell proliferation in situ under normal and pathological conditions.