miR-124靶向调控Notch 1通路影响肝癌细胞的增殖和迁移

摘 要] 目的 探索微小RNA-124（miR-124）通过Notch1信号通路对肝细胞性肝癌（HCC）细胞增殖和迁移的影响。方法 将肝癌细胞HepG2和人正常肝细胞HL7702作为受试对象。采用实时荧光定量PCR（qRT-PCR）法检测细胞中miR-124的表达。HepG2细胞转染miR-124模拟物（miR-124 mimic）后，CKK-8法检测细胞增殖活性，Transwell法检测细胞迁移能力。Western blotting检测Notch1信号通路表达。结果 在HepG2细胞中，miR-124表达低于人正常肝细胞HL7702（1.00±0.00） vs （21.32±1.02）；P < 0.05]；与对照组（无处理的HepG2细胞）相比，miR-124 mimic转染组中Hep G2细胞增殖明显降低（0.44±0.01） vs（0.21±0.01）；P < 0.05]，细胞迁移能力也降低（12.00%±2.00%） vs （5.67%±1.52%）；P < 0.05]；miR-124过表达可以明显抑制Hep G2细胞内Notch1信号通路蛋白表达（100.00%±0.00%） vs （36.46%±2.36%）；P <0.05]。结论 本研究显示，miR-124可靶向抑制Notch1表达，进而抑制HCC的增殖和迁移。

MiR-124 inhibits hepatocellular carcinoma cell proliferation and migration by modulating the Notch 1 signal pathway

Abstract objective To investigate the influence of microRNA-124 (miR-124) on the proliferation and migration of heptocellular carcinoma (HCC). Methods In this experiment, hepatocellular carcinoma cell line HepG2 and human normal liver cell line HL7702 were subjects. The expression of miR-124 was examined by qRT-PCR. The miR-124 mimics was transfected into HCC cell line HepG2 cells, and the cell proliferation and migration were examined by cell counting kit-8 (CCK-8) assay and transwell assay, respectively. The expression of Notch1 signal pathway was determined by Western blotting. Results The expression level of miR-124 in HepG2 was significantly lower than that in human normal liver cell line HL7702 (P<0.05). For HepG2 cells, after transfection, cellular proliferation and migration were inhibited by miR-124 mimic (P<0.05). Moreover, Notch1 signaling pathway was significantly inhibited by over-expression of miR-124 in HepG2 cells. ConclusionOur study suggests that miR-124 may inhibit the heptocellular carcinoma cell proliferation and migration by modulating the Notch1 signal pathway.