BCAT1对HSC-LX2人肝星状细胞纤维化相关基因表达的影响

摘要] 目的 探讨支链氨基酸转氨酶1（BCAT1）对HSC-LX2人肝星状细胞纤维化相关基因表达的影响。方法 构建携带BCAT1基因的慢病毒载体，将病毒转染HSC-LX2细胞，运用qRT-PCR和Western blotting检测转染BCAT1过表达病毒后的HSC-LX2细胞中BCAT1、ACTA2、TIMP1、MMP2和COL1A1基因的表达情况。结果 携带BCAT1基因的慢病毒转染HSC-LX2细胞后，实验组（转染p-BCAT1）HSC-LX2细胞和对照组（转染Vec）HSC-LX2细胞相比，促进纤维化的ACTA2、TIMP1、COL1A1基因的mRNA及蛋白表达均升高，抗纤维化的MMP2基因的mRNA及蛋白表达均下降，两者的差异均具有统计学意义（P < 0.05）。结论 BCAT1增强HSC-LX2人肝星状细胞中ACTA2、TIMP1、COL1A1促纤维化相关基因的表达，抑制抗纤维化相关基因MMP2的表达。

The effect of BCAT1 on the expression of fibrosis related genes in HSC-LX2 human stellate cells

Abstract objective To investigate the effect of branched-chain aminotransferases 1 (BCAT1) on expression of fibrosis-related genes in HSC-LX2 human hepatic stellate cells. Methods The lentiviral vector expressing BCAT1 gene was constructed and transduced into HSC-LX2 cells. After the exogenous expression of BCAT1 in HSC-LX2 cells, the mRNA and protein expression levels of ACTA2, TIMP1, MMP2 and COL1A1 were detected by qRT-PCR and Western blotting. Results After BCAT1 transduction, the HSC-LX2 cells in the p-BCAT1 group were compared with those in Vec group. We found that the mRNA and protein levels of ACTA2, TIMP1 and COL1A1 were up-regulated while the mRNA and protein expression levels of MMP2 were down-regulated in p-BCAT1 group. The differences were both statistically significant (P<0.05). Conclusion BCAT1 enhances the expression levels of ACTA2, TIMP1 and COL1A1 in HSC-LX2 cells which accelerate fibrosis, and inhibits the expression levels of MMP2 which restrain fibrosis.