肝细胞性肝癌组织中LVD和MVD的表达及其与肝癌侵袭转移的关系

摘要] 目的 研究肝细胞性肝癌组织中淋巴管密度（lymphatic vessel density，LVD）和微血管密度（microvessel density，MVD）在肝细胞性肝癌中的表达，及其与肝癌侵袭和转移的关系。方法 采用免疫组织化学Supervision法分别检测40例肝细胞性肝癌患者的癌组织、癌旁组织以及10例非肝癌患者的肝脏组织中LVD和MVD的表达情况，分析其与肝癌肝内转移、门脉癌栓和淋巴转移间的关系；分析癌旁组织LVD与癌组织MVD表达之间的关系。结果?在40例肝细胞性肝癌患者的癌组织、癌旁组织及10例非肝癌患者的肝脏组织中平均LVD值分别为（10.65±3.75）、 （8.98±2.52）、 （3.15±1.49）；癌组织与癌旁组织及正常组织的LVD值差异有均统计学意义（均 P ＜0.01）。癌组织、癌旁组织及正常肝脏组织平均MVD值分别为 （71.08±43.19）、 （23.20±4.70）、 （16.80±3.01），癌组织与癌旁组织及正常组织的MVD值差异有统计学意义（均 P ＜0.01）。结论 肝细胞性肝癌组织中LVD及MVD高表达，与肝内转移、门静脉癌栓及淋巴转移有关。

Study on the association of LVd and MVd expression levels in hepatocelluar carcinoma tissue with the invasiveness and metastasis of hepatocelluar carcinoma

Abstract objective To reveal the the expression of lymphatic vessel density (LVD) and microvessel density (MVD) in hepatocelluar carcinoma (HCC) tissue, and the relationship with the invasiveness and metastasis of HCC. Methods Supervision was used as an immunohistochemical technique, and the expressions of LVD and MVD were measured respectively in paracancerous and carcinoma tissues of 40 HCC patients and 10 non-HCC patients. By doing so, the relationship between the expressions and intrahepatic metastasis, portal vein thrombus, and lymphatic metastasis was analyzed. Moreover, the correlation between LVD in the paracancerous tissue and MVD in the carcinoma tissue was explored. Results It was found that the mean LDVs in the carcinoma tissues and the paracancerous tissues of the 40 HCC patients, and in the liver tissues of the 10 non-HCC patients were (10.65±3.75), (8.98±2.52), and (3.15±1.49), respectively, indicating there was a significant difference (P<0.01) between the carcinoma tissues and the paracancerous or normal liver tissues. Furthermore, the mean MVDs in the carcinoma tissues and the paracancerous tissues of the 40 HCC patients, and in the liver tissues of the 10 non-HCC patients, were (71.08±43.19), (23.20±4.70) and (16.80±3.01), respectively. A significant difference (P<0.01) was also found between the carcinoma tissues and the paracancerous tissues or normal liver tissues. Conclusion The over-expression of LVD and MVD in HCC tissues is found in this study, which may be associated with intrahepatic metastasis, portal vein thrombus, and lymphatic metastasis in liver tissues.