养殖污水有机提取物对小鼠睾丸细胞的DNA损伤作用

目的 探讨养殖污水中有机污染物的遗传毒性.方法 雄性健康昆明种小鼠按体重随机分组,每组6只;分别于春、秋季采集天津市某污水养鱼池的养殖污水,以其有机提取物原液（25.00 L/kg）、1/2原液剂量（12.50L/kg）、1/4原液剂量（6.25L/kg）经腹腔注射染毒小鼠,连续3 d;以DMSO为阴性对照,环磷酰胺为阳性对照.通过非荧光染色彗星试验检测养殖污水有机提取物所致小鼠睾丸细胞DNA损伤作用.结果 春、秋季养殖污水有机提取物各染毒剂量组小鼠睾丸细胞拖尾率和平均尾长与阴性对照组比较,差别均有统计学意义（P＜0.01,P＜0.05）,且拖尾率、平均尾长与染毒剂量之间均呈剂量-反应关系（r=0.829～0.942,P均＜0.01）.结论 养殖污水中含有可引起小鼠睾丸细胞DNA损伤的有机污染物,这些污染物有可能通过食物链对人群健康产生潜在危害.

DNA Damage in Mice Testicle Cells Induced by Organic Extracts of Fishpond Water Containing Sewage

Objective To understand the genotoxicity of organic extracts (OEs) of fishpond water containing sewage.Methods Male Kunming mice were randomly divided into different groups according to the body weight,6 in each group.The OEs were administrated to the mice through peritoneal injection at the doses of 25.00 L/kg,12.50 L/kg and 6.25 L/kg respectively for 3 consecutive days.The negative and positive control groups were treated with DMSO and cyclophosphamide (CP) respectively.The DNA damage was tested by using un-fluorescent staining comet assay.Results The tailed-cells rates and tail length in each OEs treated group were significantly higher than those in the negative control group (P<0.01 and P<0.05 ).There were significant dose-response relationships between the tailed-cells rates,tail length and the doses of OEs (r=0.829-0.942,P<0.01).Conclusion The fishpond water containing sewage may contain some organic pollutants which have potential adverse effects on human health.