广东海风藤提取物HS_2对老年痴呆小鼠的药效学研究

目的　研究广东海风藤提取物HS2 对老年痴呆 (AD)模型小鼠的治疗作用及作用机制。方法　选用昆明种小鼠 ,腹腔注射D 半乳糖和灌胃三氯化铝 90d制备老年痴呆小鼠模型 ,3个治疗组在造模的后 4 0d分别灌胃低、中、高剂量HS2 。通过Morris水迷宫、RT PCR ,常规HE染色及Aβ1～ 40免疫组化染色 ,来观察HS2 对AD模型小鼠学习记忆、脑内早老素 1(PS1)、β位点APP内切酶 (BACE)基因表达及海马、皮层中老年斑的影响。结果　低、中、高剂量的HS2 均能改善AD小鼠学习记忆 ,下调PS1和BACE基因的表达 ,减少老年斑的生成。结论　不同剂量的HS2 能改善AD小鼠的学习记忆能力 ,降低PS1,BACEmRNA的含量 ,减少脑内Aβ沉积所形成的老年斑

The therapy effect of Kadsura heteroclita on the AD model mice

Aim The pharmacodynamics and mechanism of Kadsura heteroclita on the Alzheimers disease (AD) model mice were studied. Methods The Kunming mice were treated with D-galactose and AlCl 3(90 d)to make the AD animal model. The therapy groups were treated with HS 2 (po, 40 d) respectively since d51. The Morris water-maze test, the expressions of PS1, BACE, as well as the pathological observation of the hippocampus and cerebral cortex were carried out to evaluate the effect of HS 2 on the AD model mice. Resluts By oral administration with HS 2, the capacity of learning and memory of the AD model mice was improved. HS 2 can downregulate the expression of PS1, BACE and decrease the amount of senile plaque in their brains. Conclusion HS 2 can meliorate AD model mice's learning and memory deficiency as well as decrease the mRNA content of PS1, BACE and senile plaque in their brains.