胎盘生长因子抑制剂治疗子宫内膜异位症的实验研究

目的：初步探讨胎盘生长因子（ PLGF）抑制剂对大鼠子宫内膜异位症（ EMS）的影响。方法用EMS患者分泌期子宫内膜配成组织悬液接种于雌性严重联合免疫缺陷（ SCID）小鼠腹壁以建立EMS动物模型，按照病灶面积分成2组，每组16只，实验组给予PLGF单克隆抗体50mg· kg^-1· d^-1，腹腔注射，连续注射10d；对照组给予磷酸缓冲液20mL· kg^-1· d^-1腹腔注射，连续注射10d。结果实验组异位病灶面积小于对照组（t＝3．6，P＜0．05），实验组病理学评分小于对照组（t＝16．2，P＜0．05），实验组血管密度小于对照组（t＝16．4，P＜0．05）。结论 PLGF对血管生成有抑制作用，可以用来治疗大鼠子宫内膜异位症。

Experimental study of PLGF inhibitor in treating endometriosis

Objective To investigate the effect of placental growth factor （PLGF） inhibitor on treating endometriosis （EMS）.Methods Endometriosis model was established by implanting endometrial tissue from patients into severe combined immunodeficient disease （ SCID ） mice.According to lesion area , mice were divided into PLGF group and control group , the former receiving i .p.injection of mAb against PLGF （50 mg/kg） for ten days, while the latter receiving i.p.injection of normal phosphate buffer （20 mL/kg） for ten days.These were 16 mice in both groups.Results Ectopic area in the PLGF group was significant smaller than that in the control group （t=3.6,P〈0.05）, and pathology score was lower （t=16.2,P〈0.05）.Vessel density in the PLGF group was lower than that in the control group （t=16.4, P〈0.05）.Conclusion PLGF has inhibiting effect on angiogenesis , and it can be used to treat EMS in mice .