手足口病患儿血清及脑脊液中 S100 B和 NSE 的表达与病毒性脑炎发生的相关性分析

目的：探讨手足口病患儿血清及脑脊液中S100B蛋白（S100B）和神经元特异烯醇化酶（NSE）的表达与病毒性脑炎发生的相关性。方法选择2012年2月到2013年11月黄石市妇幼保健院收治的35名手足口病并发病毒性脑炎患者为观察组，35名单纯手足口病患者为对照组，检测血清及脑脊液的S100B和 NSE含量，并进行相关性分析。结果观察组的血清中S100B （t＝6．544，P＜0．05）、NSE（t＝14．235，P＜0．05）含量及脑脊液中S100B（t＝13．982，P＜0．05）、NSE（t＝23．023，P＜0．05）含量明显高于对照组，对比差异明显。多元线性回归分析显示手足口病导致病毒性脑炎的发生与脑脊液中S100B（ OR：0．214，95％CI：0．086～0．563）、NSE（OR：6．139，95％CI：2．483～15．458）、血清中S100B（OR：0．384，95％CI：0．151-0．966）与NSE（OR：3．499，95％CI：1．422～8．621）含量都存在明显的相关性（P＜0．05）。结论手足口病患儿的血清和脑脊液中S100B及NSE含量可用来预测病毒性脑炎的发生。

Correlation between expressions of S100B and NSE in serum and cerebrospinal fluid and viral encephalitis in HFMD children

Objective To investigate the correlation between the expressions of S 100B and neuron-specific enolase （NSE） in serum and cerebrospinal fluid and viral encephalitis in children with hand , foot and mouth disease （ HFMD ） .Methods From February 2012 to November 2013 35 HFMD children with viral encephalitis were admitted in Maternal and Children Care Service Center of Huangshi .They were selected in observation group , and another 35 simple HFMD children were recruited in control group .The contents of S100B and NSE in serum and cerebrospinal fluid of all cases were detected , and correlation analysis was conducted .Results The serum S100B （t=6.544, P〈0.05）, serum NSE （t=14.235,P〈0.05）, S100B in cerebrospinal fluid（t=13.982,P〈0.05）and NSE in cerebrospinal fluid（t=23.023,P〈0.05） contents of the observation group were significantly higher than the control group .Multiple linear regression analysis showed that viral encephalitis caused by HFMD was correlated with the cerebrospinal fluid S 100B （OR：0.214,95%CI：0.086-0.563） and NSE （OR：6.139,95%CI：2.483-15.458）contents, and serum S100B（OR：0.384,95%CI：0.151-0.966）and NSE （OR：3.499,95%CI：1.422 -8.621） levels （P〈0.05）.Conclusion Detection of in S100B and NSE levels serum and cerebrospinal fluid in HFMD children can predict the occurrence of viral encephalitis .