胃癌细胞对化疗药物和α-干扰素的敏感性试验

目的　探讨人胃癌细胞株对不同化疗药物、α 干扰素及其相互配伍的敏感性。方法　向体外培养的人胃癌细胞 (MGC80 - 3)中加入不同配伍及不同浓度的化疗药物和α 干扰素 ,作用一定时间后 ,采用MTT法检测癌细胞对不同配伍药物的敏感性。结果　联合用药较单一用药的相对抑制率明显增高 ,尤其以氟尿嘧啶、丝裂霉素和表阿霉素 3药联合作用的抑制率最高 ;随药物剂量的增加 ,各组的抑制率有所增高 ,α 干扰素与化疗药物合用时 ,有协同抑制作用 ,尤其在先用α 干扰素 2 4小时后 ,再加用化疗药物 ,抑制率明显增高。结论　联合用药化疗疗效优于单一用药 ,加用α 干扰素具有协同抑制作用 ,尤以先用α 干扰素后 ,再联合用化疗药 ,疗效最佳。

Sensitivity testing of gastric cancer cells to antitumor drugs and interferon-alpha

Objective To investigate the sensitivity of human gastric cancer cell (MGC80 3) to antitumor drugs and interferon alpha and to compatibility of medicines. Methods To human gastric cancer cells cultured in vitro was added with different compatibility and concentrations of antitumor drugs and interferon alpha. After a certain time for reaction, MTT method was used to determine the antitumor sensitivity. Results There was a higher inhibition rate in the regimens of the combined use of antitumor drugs than that of the drugs used alone. The combined use of 5 Fu, MMC and E ADM showed the highest inhibition rate of the gastric cancer cells. With the increase of the drug dose, the inhibition rate was significantly increased. Interferon alpha had a synergetic effect in combination with the antitumor drugs. In particular, after 24 hours of the use of interferon alpha, antitumor drugs could significantly increase the inhibition rate. Conclusions The therapeutic effect of combined use of the antitumor drugs is superior to that of single antitumor drug. The interferon alpha has a synergetic effect in the chemotherapy of gastric cancers and a better efficiency used 24 hours before the combined chemotherapy.