成人原发性肾病综合征T细胞亚群、NK细胞及B细胞的变化及临床意义

目的探讨成人原发性肾病综合征（primarynephrotiesyndrome，PNS）患者T淋巴细胞亚群、NK细胞及B淋巴细胞的变化及临床意义。方法应用流式细胞仪检测64例成人PNS活动期患者外周血中T淋巴细胞亚群（CD3＋、CD3＋CD4＋、CD3＋CD8＋、CD4＋／CD8＋）、CD16＋CD56＋NK细胞及CD19＋B淋巴细胞水平的变化，同时检测30例同期来院体检的正常人作为对照。结果PNS组与对照组相比：CD3＋、CD3＋CD4＋T淋巴细胞降低．CD3＋CD8＋T淋巴细胞升高，CD4＋／CD8＋比值显著下降且差异均有统计学意义（P均〈0．05）；PNS组CD16＋CD56＋NK细胞较对照组降低，而CD19＋B淋巴细胞显著升高，且差异亦均具有统计学意义（P均〈0．05）。结论成人PNS活动期T细胞亚群的比例结构发生严重失衡，细胞免疫功能降低，而体液免疫功能亢进。

The change and clinical significance of T lymphocyte subset, natural killer cell and B lymphocyte in adults with primary nephrotic syndrome

Objective To investigate the change and clinical significance of T lymphocyte subset, natural killer cell and B lymphocyte in adults with primary nephrotic syndrome（PNS）. Methods 64 cases PNS patients in acute phase and 30 cases healthy controls were selected. The change of T lymphocyte subset （CD3＋; CD3＋CD4＋; CD3＋CDS＋; CD4＋/CD8＋ ）, natural killer cell （CD16＋CD56＋） and B lymphocyte （CD19＋） in peripheral blood were measured by flow cytometry. Results The percentages of CD3＋ T lymphocyte, CD3＋CD4＋ T lymphocyte and the ratio of CD4＋/CD8＋ were significantly lower in PNS group than that in control group, while the level of CD3＋CD8＋ T lymphocyte was greatly higher in PNS group than that in control group and the differences all had statistical significance （Pall〈 0.05）. Meanwhile, compared with control group, the CD16＋CD56＋ natural killer cell depressed, but the CD19＋ B lymphocyte rised in PNS group and the differences all had statistical significance （Pall〈 0.05）. Conclusion The serious imbalance of the proportion of T lymphocyte subset exist in acute phase PNS patients meanwhile with the cellular immune function depressed and the humoral immune function strengthened.