慢性HBV感染者血清HBVDNA载量与HBeAg／抗-HBe、ALT之间的关系

目的探讨慢性HBV感染者血清HBVDNA载量与HBeAg／抗-HBe以及丙氨酸转氨酶（ALT）水平之间的关系。方法采用酶联免疫吸附法（ELISA）检测134例慢性HBV感染者HBV血清标志物，根据HBeAg／抗-HBe检测结果将患者分为Ⅰ组（HBeAg＋／抗-HBe-，43例）；11组（HBeAg-／抗-HBe＋，69例）；Ⅲ组（HBeAg-／抗-Hbe-，22例）。同时应用实时荧光定量PCR方法以及连续监测法检HBVDNA载量以及ALT水平。结果Ⅰ组HBVDNA阳性率和HBVDNA载量明显高于Ⅱ、Ⅲ组（P〈0．01）。HBVDNA阳性患者ALT异常率高于HBVDNA阴性患者（P〈0．01）。HBVDNA阳性患者HBVDNA载量与ALT水平之间无相关性（r=0．174，P=0．156）。结论血清HBVDNA载量与HBeAg／抗-HBe以及ALT之间存在一定的关系，但不能单纯依靠HBeAg／抗-HBe以及ALT来判断HBV在体内的复制情况，三项指标联合检测对HBV慢性感染者的病情检测、治疗具有重要意义。

The correlation among HBV DNA load, HBeAg/Anti-HBe and Alanine Aminotransferase in patients with chronic hepatitis B infection

Objective To explore the relationship among HBV DNA load, HBeAg/Anti-HBe and ALT in patients with chronic hepatitis B infection. Methods HBV markers in 134 patients with chronic HBV infection were detected by ELISA,and patients were divided into group Ⅰ (HBeAg +/Anti-HBe-,43 patients) ,group Ⅱ (HBeAg-/Anti-HBe + ,69 patients)and group Ⅲ (HBeAg-/Anti-Hbe-,22 patients)according to the results of presence of HBeAg/Anti-HBe. HBV DNA load and ALT were tested respectively by fluorescence quantitative polymerase chain reaction technology and successive monitor reaction. Results Both the positive rate and the HBV DNA load quantification in group Ⅰ were higher than in group Ⅱ and Ⅲ (P＜0.01). Abnormality rate of ALT in HBV DNA positive patients was higher than the patients with HBV DNA negitive(P＜0.01). There was no relationship between HBV DNA load and ALT in HBV DNA positive patients. Conclusion There was a certain corelation among HBV DNA load, HBeAg/Anti-HBe and ALT,but the active degree of HBV replication could not to be assessed by HBeAg/Anti-HBe or ALT alone. It was necessary for the padient with chronic HBV infection to examine them together.