Recombinant human bone morphogenetic protein induces bone formation. |
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| Authors: | E A Wang V Rosen J S D'Alessandro M Bauduy P Cordes T Harada D I Israel R M Hewick K M Kerns P LaPan et al. |
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| Affiliation: | Genetics Institute, Cambridge, MA 02140. |
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| Abstract: | We have purified and characterized active recombinant human bone morphogenetic protein (BMP) 2A. Implantation of the recombinant protein in rats showed that a single BMP can induce bone formation in vivo. A dose-response and time-course study using the rat ectopic bone formation assay revealed that implantation of 0.5-115 micrograms of partially purified recombinant human BMP-2A resulted in cartilage by day 7 and bone formation by day 14. The time at which bone formation occurred was dependent on the amount of BMP-2A implanted; at high doses bone formation could be observed at 5 days. The cartilage- and bone-inductive activity of the recombinant BMP-2A is histologically indistinguishable from that of bone extracts. Thus, recombinant BMP-2A has therapeutic potential to promote de novo bone formation in humans. |
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