重组人血管内皮抑素联合顺铂治疗小鼠肺转移癌实验研究

目的:探讨重组人血管内皮抑素(恩度)联合顺铂抑制小鼠肺转移癌的作用和机制。方法:取H22肝癌细胞株腹水瘤反复从小鼠尾静脉注入,建立高肺转移小鼠模型,随机分为4组:生理盐水、顺铂、恩度和顺铂与恩度联合治疗组,10只/组。从尾静脉接种后第7天腹腔内给药,1次/d,连用14 d,第21天处死,解剖出小鼠肺脏,称质量,计数肺转移结节数,并对肺转移瘤组织进行免疫组化染色,检测血管内皮生长因子(VEGF)表达,计数微血管密度。结果:生理盐水组、恩度组、顺铂组和联合治疗组肺转移率分别为100%、70%、60%和40%,肺转移结节数分别为(28.6±10.2)、(20.7±5.6)、(14.3±6.4)和(8.9±3.8)个,微血管密度计数为(31.4±3.1)、(24.6±5.9)、(21.2±4.7)和(15.2±3.1),各治疗组结果与生理盐水组比较差异均有统计学意义(P<0.05),联合组与顺铂组或恩度组比较也具有差异(P<0.05)。生理盐水组肺转移瘤组织的VEGF表达明显高于各治疗组(P<0.05)。结论:恩度单用或联合顺铂均能显著抑制H22肝癌肺转移组织的血管生成,降低了肺转移。

Combination of recombinant human endostatin and DDP for treatment of murine tumor lung metastases

OBJECTIVE: To investigate the inhibitory effects of recombinant human endostatin with DDP on the lung metastatic tumor of mice. METHODS: To obtain the highly pulmonary metastatic Kunming mice injected with H22 hepatic cells from tail vein. Forty highly pulmonary metastatic mice were divided into four groups (n=10) to receive the injection of physiological saline, endostatin, DDP and endostatin plus DDP, receptively for fourteen days-seven days after the inoculation. On the 21st day, all the mice were scarif...