| RNAi沉默CD133基因表达对结肠癌细胞株HT-29影响的研究 |
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| 引用本文： | 唐茂盛,王伟军,彭欢,姚厚山,胡志前.RNAi沉默CD133基因表达对结肠癌细胞株HT-29影响的研究[J].中华肿瘤防治杂志,2011,18(12):936-939. |
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| 作者姓名： | 唐茂盛 王伟军 彭欢 姚厚山 胡志前 |
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| 作者单位： | 第二军医大学附属长征医院普外科,上海,200003 |
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| 基金项目： | 上海科委科研计划项目(08140902900) |
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| 摘 要： | 目的:探讨通过RNA干扰沉默CD133基因对结肠癌细胞株HT-29的增殖和侵袭力的影响。方法:将CD133siRNA用脂质体转染入HT-29细胞,以逆转录-聚合酶链反应(RT-PCR)检测CD133mRNA的变化,蛋白质印迹法检测转染前后蛋白的表达水平,用MTT比色法和Transwell小孔实验分别检测癌细胞的增殖及侵袭力变化。结果:CD133siRNA转染成功,与正常对照组相比,转染组CD133mRNA及蛋白的表达水平均有明显的抑制,并呈现浓度时间依赖性。MTT实验证明转染组细胞随时间延长,增殖逐渐受到抑制,P<0.05。Tran-swell小孔实验中经转染处理的穿膜细胞数明显减少(P<0.05),RNA干扰降低了细胞的侵袭性。结论:CD133在HT-29癌细胞增殖及侵袭过程中起重要作用,RNA干扰可以有效抑制其CD133的表达,并能有效降低癌细胞的增殖及侵袭力。
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| 关 键 词： | 大肠肿瘤 RNA干扰 基因 CD133 基因治疗 HT-29细胞 |
Inhibition of CD133 by RNA Interference in human colon carcinoma cell line HT-29 |
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| TANG Mao-sheng,WANG Wei-jun,PENG Huan,YAO Hou-shan,HU Zhi-qian.Inhibition of CD133 by RNA Interference in human colon carcinoma cell line HT-29[J].Chinese Journal of Cancer Prevention and Treatment,2011,18(12):936-939. |
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| Authors: | TANG Mao-sheng WANG Wei-jun PENG Huan YAO Hou-shan HU Zhi-qian |
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| Institution: | TANG Mao-sheng,WANG Wei-jun,PENG Huan,YAO Hou-shan,HU Zhi-qianDepartment of General Surgery,Changzheng Hospital,Second Military Medical University,Shanghai 200003,P.R.China |
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| Abstract: | OBJECTIVE: To construct an expression plasmid of siRNA against CD133 gene and to investigate its biological behavioral effect on human colon carcinoma cell line HT-29.METHODS: we inhibited the CD133 expression using the siRNA method.Expression levels of CD133 mRNA and protein from HT-29 after transfection were examined by RT-PCR and Western blot respectively.Changes of the cell growth activity in response to transfected plasmid were evaluated by MTT assay.The migration ability of transfected HT-29 cells wer... |
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| Keywords: | colon neoplasms siRNA gene CD133 gene therapy HT-29 cells |
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