| 高效液相色谱法测定硫鸟嘌呤的含量及有关物质 |
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| 引用本文： | 邓祖跃,倪维芳,辛艳飞,金瓯. 高效液相色谱法测定硫鸟嘌呤的含量及有关物质[J]. 中国药学杂志, 2009, 44(13): 1029-1032 |
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| 作者姓名： | 邓祖跃 倪维芳 辛艳飞 金瓯 |
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| 作者单位： | 1.浙江省食品药品检验所 杭州 310004;2.浙江省医学科学院 杭州 310013 |
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| 基金项目： | 国家药典委员会2010版药典专项基金(国药典化发〔2008〕97号) |
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| 摘 要： | 目的建立高效液相色谱法测定硫鸟嘌呤原料及片剂的含量及有关物质。方法采用Altima C18色谱柱,以0.05mol·L-1磷酸二氢钠溶液(pH3.0)为流动相,柱温为35℃;检测波长248nm;流速1.0mL·min-1。结果硫鸟嘌呤在8～120mg·L-1内线性关系良好(r=0.9999),最低检测限为0.12mg·L-1,回收率为99.27%。结论本方法准确、灵敏,精密度高、专属性强,可用于硫鸟嘌呤含量及有关物质的测定。
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| 关 键 词： | 高效液相色谱法 硫鸟嘌呤 含量测定 有关物质 |
| 收稿时间： | 2008-12-03; |
Determination of Tioguanine and Its Related Substances by HPLC |
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| DENG Zu-yue,NI Wei-fang,XIN Yan-fei,JIN Ou. Determination of Tioguanine and Its Related Substances by HPLC[J]. Chinese Pharmaceutical Journal, 2009, 44(13): 1029-1032 |
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| Authors: | DENG Zu-yue NI Wei-fang XIN Yan-fei JIN Ou |
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| Affiliation: | 1. Zhejiang Institute for Food and Drug Control, Hangzhou 310004，China; 2. Zhejiang Academy of Medical Sciences, Hangzhou 310013，China |
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| Abstract: | OBJECTIVE To establish a reversed-phase high performance liquid chromatography method for the determination of the thioguanine and its related substances.METHODS The analytical column was Altima C18 and the mobile phase was 0.05 mol·L-1 sodium dihydrogen phosphate acid(pH 3.0).The column temperature was maintained at 35 ℃.The detection wavelength was 248 nm.The flow rate was 1.0 mL·min-1.RESULTS Thioguanine was linear in the range from 8 to 120 mg·L-1(r=0.999 9).The limit of detection was 0.12 mg·L-1.The average recoveries were 99.27%.CONCLUSION The method was proved to be accurate, sensitive and precise and showed highly specific.It can be applied for the content assay, as well as the related substances of thioguanine |
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| Keywords: | HPLC thioguanine assay related substance |
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