LC-MS/MS法测定大鼠口服人参皂苷Rg1,Re,Rb1和Rd的药动学

 目的建立检测大鼠血浆中人参皂苷Rg₁,Re,Rb₁和Rd的分析方法,并研究这4种成分在大鼠体内药动学特征。方法采用LC-MS/MS测定4种人参皂苷的血药浓度,用DAS2.0软件对结果进行统计分析。结果质谱采用MRM方式进行检测,人参皂苷Rg1和Rb1的线性范围为2～1000μg·L^-1,相关系数为0.997和0.998,人参皂苷Re的线性范围为1～1000μg·L_-1,相关系数为0.998,人参皂苷Rd的线性范围为1.6～1000μg·L^-1,相关系数为0.9940。这4种成分的最低检测限分别为2,1,2和1.6μg·L^-1,提取回收率为75.69%到98.79%,方法的精密度、准确度和稳定性均符合要求。结论本方法操作简单、专属性强、灵敏度高、准确性好,可用于人参皂苷类成分的体内测定。

Pharmacokinetic Studies of Ginsenoside Rg1,Re,Rb1 and Rd in Rats by LC-MS/MS Method

OBJECTIVE To develop a sensitive and reliable method for the quantification of pharmacokinetic study of Rg₁,Re,Rb₁ and Rd in rat plasma and to study their pharmacokinetic characteristics in rat.METHODS The concentrations of Rg₁,Re,Rb₁ and Rd in rat plasma were determined by a HPLC-MS/MS method after oral administration of total ginsenosides.The pharmacokinetic data were analyzed by DAS 2.0 software.RESULTS The analytes were determined by electrospray positive ionization mass spectrometry in the MRM mode.The standard curves for R₁,Re,Rb₁ and Rd were linear over the concentration range of 2-1 000,2-1 000,1-1 000 and 1.6-1 000 μg·L^-1 respectively.The lower limits of quantification were 2,1,2 and 1.6 μg·L^-1 for R₁,Re,Rb₁ and Rd respectively.The precisions,the accuracy and the stability all met the requirements for all analytes.CONCLUSION The method was sensitive,accurate,rapid and can be applied to determine the plasma concentration of Ginsenoside Rg₁,Re,Rb₁ and Rd.