山茱萸炮制前后的HPLC指纹图谱研究

 目的建立山茱萸炮制前后的高效液相(HPLC)指纹图谱。方法选择山茱萸生、制品各12批,以体积分数80%甲醇溶液超声提取制备溶液,应用Agilent Zorbax C₁₈(4.6mm×250mm,5μm)柱,二极管阵列检测器(DAD),以0.1%磷酸溶液和乙腈为流动相,梯度洗脱,流速1mL·min^-1,柱温30℃,检测波长240nm。结果山茱萸生品样品中共检测到19个共有色谱峰,可以指认5个共有峰;山茱萸制品样品中共检测到23个共有色谱峰,可以指认6个共有峰。结论从整体上显示生、制品的特征,为山茱萸生、制品的质量控制提供有效手段。

Study on Fingerprint of Crude and Processed Cornus officinalis by HPLC

OBJECTIVE To establish the fingerprint of crude and processed Cornus officinalis by HPLC. METHODS The 12 batches of crude drug and 12 batches of processed products were extracted in 80% methanol ultrasonic extraction, respectively. Subsequently, the samples solution was separated on an Agilent Zorbax C₁₈ column (4.6 mm×250 mm, 5 μm) and detected by DAD.Mobile phase was composed of aqueous phosphoric acid (0.1%) and acetonitrile using a gradient elution. Analysis was performed at 30 ℃ with a flow rate of 1 mL·min^-1 and UV detection at 240 nm. RESULTS There were 19 common peaks in crude drug and 5 peaks identified. There were 23 common peaks in processed products and 6 peaks identified. CONCLUSION The fingerprint of crude and processed Cornus officinalis could be applied to the intrinsic quality control of crude and processed Cornus Officinalis.