UPLC-MS/MS测定降糖类中药制剂及保健品中8种化学药的研究

 目的建立一种测定降糖类中药制剂及保健品中违禁添加8种化学药的超高效液相-串联四级杆质谱方法。方法使用超高效液相-串联四极杆质谱的多反应监测(MRM)模式测定。结果8种化学药测定的线性范围在0.5～200.0μg·L^-1之间,r分别在0.9971～0.9998之间;盐酸二甲双胍、盐酸苯乙双胍、格列奇特、盐酸吡格列酮、格列吡嗪、格列美脲、格列苯脲和格列喹酮的检出限分别为5.2,1.1,5.4,0.2,5.1,0.2,0.7和3.1μg·kg^-1;3个水平的回收率分别在84.3%～121.1%间。结论该方法快速、准确、灵敏,可用于降糖类中药制剂及保健品中违禁添加8种化学药的测定。

Stuyies on Eight Anti-iabetic Medicines in Traditional Chinese Medicines and Health Foods by UPLC MS/MS

OBJECTIVE To establish a method for the determination of 8 anti-iabetic medicines in traditional Chinese medicines and health foods by UPLC triple-quadrupole tandem mass spectrometer.METHODS Using UPLC-MS/MS to determinate by multi-reactions monitoring(MRM) technology.RESULTS The standard curves of the 8 chemical compounds showed good linearity over the concentration range of 0.5-200 μg·L^-1 and the r between 0.997 1 and 0.999 8 respectively.The detection limits of the methformin hydrochloride,phenformin hydrochloride,gliclazide,pioglitazone hydrochloride,glipzide,glimepiride,glibenclamide and gliquidone were 5.2,1.1,5.4,0.2,5.1,0.2,0.7,3.1 μg·kg^-1 separately.The average recoveries were 84.3%-121.1% at three levels.CONCLUSION The method had the advantages of rapidness,higher precision and higher sensitivity,was suitable for the determination of 8 anti-iabetic medicines in traditional Chinese medicines and health foods.