| 单克隆荧光偏振免疫法、多克隆荧光偏振免疫法和高效液相色谱法分别测定环孢素A血药浓度结果的相关性探讨 |
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| 引用本文： | 刘晓,张相林,张镭,李朋梅,李凯鹏,卢炜.单克隆荧光偏振免疫法、多克隆荧光偏振免疫法和高效液相色谱法分别测定环孢素A血药浓度结果的相关性探讨[J].中国药学杂志,2009,44(10):790-793. |
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| 作者姓名： | 刘晓 张相林 张镭 李朋梅 李凯鹏 卢炜 |
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| 作者单位： | 1.北京大学药学院 北京 100191;2.卫生部中日友好医院药学部 北京 100029;3.天然药物及仿生药物国家重点实验室（北京大学） 北京 100191 |
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| 摘 要： | 目的本实验定量比较当前通行的3种CsA(环孢素A)测定方法,考察方法间相关性,建立普遍适用的测定结果换算方法。方法分别采用高效液相色谱法、单克隆和多克隆荧光免疫法测定样品,测定结果采用Levene和Tamhane's T2进行方差分析,用Spearman进行相关性比较。结果经过统计分析得到3组方法结果互换方程式,YmFPIA=0.967 1XHPLC+45.761; YpFPIA=1.493 7XHPLC+205.94;YpFPIA=1.563XmFPIA+129.72。结论高效液相色谱法、单克隆和多克隆荧光偏振免疫法三者间具有良好的相关性,通过方程式可将各自测定结果方便准确的互算。
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| 关 键 词： | 环孢素A 高效液相色谱法 单克隆荧光偏振免疫法 多克隆荧光偏振免疫法 相关性 |
| 收稿时间： | 2009-01-22; |
A Validation Study of Three Selected Methods Routinely Used For Measurement of Cyclosporine A |
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| LIU Xiao,ZHANG Xiang-lin,ZHANG Lei,LI Peng-mei,LI Kai-peng,LU Wei,.A Validation Study of Three Selected Methods Routinely Used For Measurement of Cyclosporine A[J].Chinese Pharmaceutical Journal,2009,44(10):790-793. |
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| Authors: | LIU Xiao ZHANG Xiang-lin ZHANG Lei LI Peng-mei LI Kai-peng LU Wei |
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| Institution: | 1. Peking University School of Pharmaceutical Science，Beijing 100191，China；2. Pharmacy Department，Sino-Japan Friendship Hospital，Beijing 100029，China；3. State Key Laboratory of Natural and Biomimetic Drugs (Peking University)，Beijing 100191，China |
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| Abstract: | OBJECTIVE To compare three methods.HPLC,monoclonal fluorescence polarization immunoassay(mFPIA) and polyclonal fluorescence polarization immunoassay(pFPIA),routinely used for measuring cyclosporine A(CsA) in patients whole blood.METHODS The data obtained by the three methods were analyzed using Levene and Spearman method. RESULTS Data by the three methods showed good correlation.The three equations were as follows:YmFPIA=0.967 1XHPLC+ 45.761;FpFPIA=1.493 7Xhplc+205.94;YpFPIA=1.563XmFPIA+129.72.CONCLUSION The results of the three methods can be interconverted easily. |
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| Keywords: | cyclosporine A HPLC mFPIA pFPIA correlation |
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