| 壳聚糖及其衍生物对HaCaT细胞膜电位的影响 |
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| 引用本文： | 何文,肖礼海,孙安琪,王小芹,徐燃. 壳聚糖及其衍生物对HaCaT细胞膜电位的影响[J]. 中国药学杂志, 2009, 44(14): 1063-1066 |
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| 作者姓名： | 何文 肖礼海 孙安琪 王小芹 徐燃 |
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| 作者单位： | 1.武汉大学人民医院药学部 武汉 430060;2.武汉大学药学院 武汉 430072;3.中南民族大学生命科学学院 武汉 430074 |
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| 基金项目： | 湖北省自然科学基金资助项目(2005ABA107) |
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| 摘 要： | 目的用流式细胞仪检测壳聚糖及其衍生物对HaCaT细胞膜电位的影响。方法合成不同取代度的N-三甲基壳聚糖(TMC)和N-羧甲基壳聚糖(MCC);用纯水作对照,运用荧光分子探针DiBAC4(3)标记HaCaT细胞膜电位,流式细胞仪检测壳聚糖及其衍生物处理之后细胞膜电位的变化情况。结果与纯水组比较,壳聚糖及其衍生物都能显著降低细胞膜电位,其中,TMC20组作用最明显,TMC40组作用最微弱。结论壳聚糖及其衍生物降低细胞膜电位,可能是其具备透皮吸收促进作用的原因之一。
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| 关 键 词： | 壳聚糖 壳聚糖衍生物 HaCaT细胞 细胞膜电位 |
| 收稿时间： | 2008-08-01; |
Effect of Chitosan and Its Derivatives on Membrane Potential of HaCaT Cells |
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| HE Wen,XIAO Li-hai,SUN An-qi,WANG Xiao-qin,XU Ran. Effect of Chitosan and Its Derivatives on Membrane Potential of HaCaT Cells[J]. Chinese Pharmaceutical Journal, 2009, 44(14): 1063-1066 |
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| Authors: | HE Wen XIAO Li-hai SUN An-qi WANG Xiao-qin XU Ran |
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| Affiliation: | 1. Department of Pharmacy， Renmin Hospital， Wuhan University， Wuhan 430060， China; 2. Department of Pharmacy， Wuhan University， Wuhan 430072， China；3. Department of Life Science, South-Central University for Nationalities, Wuhan 430074, China |
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| Abstract: | OBJECTIVE To study the effect of chitosan and its derivatives on membrane potentials of HaCaT cells using flow cytometer. METHODS N-trimethyl chitosan (TMC) with different degree of quaternization and N-carboxymethylchitosan (MCC) were synthesized from chitosan. HaCaT cells were fluorescent labeled with DiBAC4(3) and the changes of membrane potentials were measured by flow cytometer. RESULTS Compared with pure water, all of chitosan and its derivatives decreased HaCaT cells membrane potentials significantly,and the effect of TMC20 was the most significant while that of TMC40 was the weakest. CONCLUSION The decrease effect of chitosan and its derivatives on HaCaT cells membrane potentials might be one of reasons of their skin penetration enhancment. |
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| Keywords: | chitosan chitosan derivates HaCaT cells membrane potentials |
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