重组人红细胞生成素对缺氧缺血新生大鼠脑细胞的影响

目的　探讨重组人红细胞生成素 (rhEPO)对新生大鼠缺氧缺血性脑损伤 (HIBD)脑细胞凋亡的影响及rhEPO能否通过血脑脊液屏障。方法　选用Wistar大鼠 80只随机分为 4组 ,即正常对照组、HIBD组、川芎嗪 (TMP)治疗组、rhEPO治疗组 ,每组各 2 0只。制备HIBD模型后腹腔内注射rhEPO及TMP。在缺氧缺血后48h处死 ,取脑组织常规固定 ,切片行HE及原位末端标记法染色 ,测定脑细胞凋亡百分率及凋亡面密度。结果　HIBD组凋亡百分率、凋亡面密度均明显高于正常对照组 ,差异有显著性 (P均 <0 .0 1) ;rhEPO组测定值明显低于HIBD组 (P均 <0 .0 1) ,而与TMP组相比无差异 (P均 >0 .0 5)。结论　rhEPO能抑制脑细胞凋亡 ,具有与TMP类似作用 ,对HIBD有防治作用

Effects of rherythropoietin on neonatal rats with hypoxia-ischemia

Objective To assess the effect of rherythropoietin(rhEPO),on cerebrocelluar apoptosis of neonatal rats with hypoxic ischemic brain damage(HIBD).Methods Select 80 Wistar rats and divided them into 4 groups randomly;normal control group,HIBD group,the TMP treated group,rhEPO treated group,intraperitoneally injected rhEPO and TMP after established HIBD model,then rats were killed and to be routine fixed after hypoxia ischemia for another 48 hours.Apoptotic rate and apoptotic dense of rats were detected after the brain tissue were dyed with HE and Tunel.Results Apoptotic rate and apoptotic dense in HIBD group were much higher than those in normal control group( P <0.01),there were much lower in rhEPO group than HIBD group( P <0.01).There were no significant difference compared with TMP group.Conclusion rhEPO can inhibit cerebrocelluar apoptosis.rhEPO have the same effect as TMP in treating HIBD,which take a role in preventing and treating HIBD.