海洋细菌201721抗菌物质的发酵优化与性质初步研究

目的海洋细菌201 72l能产生对大肠杆菌有强烈抑制作用的活性物质,本文对其发酵条件进行优化,并对发酵产物性质进行研究。方法采用Plackett-Burman设计筛选出培养基pH、蛋白胨的量和培养温度等关键因素;继而采用响应曲面法关键因素进行优化。结果pH 6.3,蛋白胨的量8.2g,温度31.6℃为发酵的最优奈件,发酵所产生的抗菌活性物质极性中等.具有较好的热稳定性和酸碱稳定性。结论本研究所采用的条件优化方法.适用于菌株发酵条件的优化。

Fermentation conditions and characteristics of antibiotic active substances by marine bacteria 201721

Objective Marine bacteria 201721 can produce strongly inhibitive active substances against E. coli. . Its fermentation conditions and chemical properties were studied. Methods Seven factors including glucose, yeast extract, peptone, medium pH, inoculum, agitation, and culture temperature were investigated by Plackett-Burman Design, and the significant factors including medium pH, peptone and culture temperature were optimized by using Response Surface Methodology. Results The predicted maximum inhibition zone (26. 5cm) were obtained under the optimized fermentation conditions (the medium pH, peptone and culture temperature were set at 6. 3 , 8. 2 g and 31. 6 C , respectively). The antibiotic active substances have better heat and pH stabilities and middle polarity via physicochemical experiments. Conclusion The proposed approach based on Plackett-Burman and Response Surface Methodology was suitable to optimize the fermentation condition of the strain.