小鼠出生后肾脏发育过程中的细胞增殖与凋亡

目的：观察小鼠出生后肾脏发育过程中增殖细胞核抗原(PCNA)的表达及细胞凋亡的特征,探讨出生后小鼠肾脏发育过程中细胞增殖与凋亡的规律及其关系。方法：应用免疫组织化学技术和原位末端标记法(TUNEL法)分别检测小鼠出生后1～70d肾脏中PCNA阳性的细胞和凋亡细胞。结果：小鼠出生后1～70d,皮质中的肾小体、肾小管、髓放线以及髓质中的肾小管和集合管的细胞,早期增殖活跃,随着肾脏发育成熟而表达逐渐减弱。同时,也存在着细胞凋亡现象,且凋亡高峰一般出现在增殖高峰之后。结论：细胞增殖与凋亡在小鼠生后肾脏发育的整个过程中普遍存在,生后1～7d细胞增殖旺盛,增殖高峰之后出现凋亡高峰,生后28～70d两者活动均减弱。

Cell proliferation and apoptosis in mouse kidney during postnatal development

Objective:To observe the expression of proliferating cell nuclear antigen (PCNA) and the cell apopto- sis,so as to investigate the activity of cell proliferation and apoptosis in the mouse kidney during postnatal devel- opment.Methods:The expression of PCNA and apoptosis were examined in the mouse kidney 1-70 days after birth by immunohistoehemical technique and TdT-mediated dUTP nick end labelling (TUNEL).Results:The ac- tivity of cell proliferation was prominent after birth.At the early stage the PCNA-positive cells were abundant. Proliferative activity tended to decrease gradually as the renal developmental stage proceeded and eventually var- nished.At the same time,apoptotic cells were presented.The peak of cell death was later than the peak of cell proliferation.Conclusion:Cell proliferation and apoptosis exist widely in the mouse kidney during postnatal de velopment.The positive rate of PCNA is high 1-70 days after birth,and the peak of apoptosis is later than that of cell proliferation.Both of apoptosis and proliferation are weak 28-70 days after birth.