胰腺癌患者围手术期T细胞亚群和NK细胞活性的动态观察

目的探讨胰腺癌患者围手术期外周血T细胞亚群、NK细胞的变化及其临床意义.方法采用流式细胞术法分别测定58例胰腺癌患者手术前后外周血T细胞亚群CD4 、CD8 和NK细胞的水平,并与健康对照组比较分析.结果胰腺癌组治疗前外周血CD4 、CD4 /CD8 和NK细胞均降低,而CD8 增高,与对照组比较差异有显著性(P<0.01);且与淋巴结转移和肿瘤临床TNM分期有显著相关性.行根治性手术组术后CD4 、CD4 /CD8 和NK细胞均有显著性增高(P<0.05),CD8 显著性降低(P<0.05);而姑息性手术组CD4 、CD4 /CD8 和NK细胞则无显著性增高(P>0.05),CD8 无显著性降低(P>0.05).结论胰腺癌患者血清T细胞亚群和NK细胞的变化与肿瘤的浸润转移和病程有关,检测胰腺癌患者血清T细胞亚群和NK细胞的变化,有助于评估患者的疗效和预后.

Dynamic study of T lymphocyte subset and NK cell activity during perioperative period in patients with pancreatic cancer

Objective To investigate the changes and clinical significance of T lymphocyte subset and NK cell activity in peripheral blood of the patients with pancreatic cancer before and after operation.Methods The levels of T lymphocyte subset and NK cell activity in 58 patients with pancreatic cancer were detected by flow cytometry before and after opreration respectively and compared with those in healthy controls.Results The levels of CD4~+,CD4~+/CD8~+ and NK cell activity in the patients with pancreatic cancer before operation were significantly lower than those in control group(P<0.01),while the level of CD8~+ was significantly higher(P<0.01). There was significant correlation of the levels with lymph node metastasis and clinical stage (TNM).The levels of CD4~+,CD4~+/CD8~+ and NK cell activity were significantly increased in patients with pancreatic cancer after radical resection(P<0.05), while the level of CD8~+ was significantly decreased(P<0.01).But there was no significant changes in the levels of CD4~+,CD4~+/CD8~+,CD8~+ and NK cell activity in patients with pancreatic cancer after palliative operation(P>0.05).Conclusion The levels of T lymphocyte subset and NK cell activity are closely related to tumor invasion,metastasis and clinical stage (TNM).Dynamic determination of the levels of T lymphocyte subset and NK cell activity may be an important index for evaluation of tumor invasion,metatasis,therapeutic efficacy and prognosis in the patients with pancreatic cancer.