Low-cost,chromatic confocal endomicroscope for cellular imaging in vivo |
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| Authors: | Nachiket Kulkarni Andrew Masciola Abhinav Nishant Kyung-Jo Kim Heejoo Choi Arthur Gmitro Esther E. Freeman Aggrey Semeere Miriam Nakalembe Dongkyun Kang |
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| Affiliation: | 1.James C. Wyant College of Optical Sciences, University of Arizona, Tucson, Arizona 85721, USA;2.Department of Biomedical Engineering, University of Arizona, Tucson, Arizona 85721, USA;3.Department of Dermatology, Massachusetts General Hospital, Boston, MA 02114, USA;4.Infectious Diseases Institute, Makerere University College of Health Sciences, Kampala, Uganda;5.Department of Epidemiology and Biostatistics, University of California at San Francisco, San Francisco, CA 94143, USA |
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| Abstract: | We have developed a low-cost, chromatic confocal endomicroscope (CCE) that can image a cross-section of the tissue at cellular resolution. In CCE, a custom miniature objective lens was used to focus different wavelengths into different tissue depths. Therefore, each tissue depth was encoded with the wavelength. A custom miniature spectrometer was used to spectrally-disperse light reflected from the tissue and generate cross-sectional confocal images. The CCE prototype had a diameter of 9.5 mm and a length of 68 mm. Measured resolution was high, 2 µm and 4 µm for lateral and axial directions, respectively. Effective field size was 468 µm. Preliminary results showed that CCE can visualize cellular details from cross-sections of the tissue in vivo down to the tissue depth of 100 µm. |
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