Deriving high contrast fluorescence microscopy images through low contrast noisy image stacks |
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Authors: | Sebastian Acuñ a,Mayank Roy,Luis E. Villegas-Herná ndez,Vishesh K. Dubey,Balpreet Singh Ahluwalia,Krishna Agarwal |
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Affiliation: | 1.Department of Physics and Technology, UiT The Arctic University of Norway, 9010 Tromsø, Norway;2.Indian Institute of Technology (Indian School of Mines), Dhanbad 826004, India;3.Shared co-authors |
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Abstract: | Contrast in fluorescence microscopy images allows for the differentiation between different structures by their difference in intensities. However, factors such as point-spread function and noise may reduce it, affecting its interpretability. We identified that fluctuation of emitters in a stack of images can be exploited to achieve increased contrast when compared to the average and Richardson-Lucy deconvolution. We tested our methods on four increasingly challenging samples including tissue, in which case results were comparable to the ones obtained by structured illumination microscopy in terms of contrast. |
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