| siRNA封闭BRCAA1基因对乳腺癌细胞MCF-7增殖和Rb基因表达的影响 |
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| 引用本文： | 杨 浩,崔大祥,李 清,黄 拓,高 峰,贺 蓉,潘碧峰,邵 君,尤晓钢,刘风涛. siRNA封闭BRCAA1基因对乳腺癌细胞MCF-7增殖和Rb基因表达的影响[J]. 中国肿瘤生物治疗杂志, 2006, 13(3): 181-184 |
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| 作者姓名： | 杨 浩 崔大祥 李 清 黄 拓 高 峰 贺 蓉 潘碧峰 邵 君 尤晓钢 刘风涛 |
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| 作者单位： | 上海交通大学微纳科学和技术研究院,微纳制造技术国家重点实验室,薄膜与微纳技术教育部重点实验室,生物纳米科学与工程研究室,上海,200030 |
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| 基金项目： | 国家重点基础研究发展计划(973计划);中国科学院资助项目 |
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| 摘 要： | 目的：〖HT5"SS〗 研究siRNA封闭乳腺癌抗原相关基因1(BRCAA1)对乳腺癌细胞MCF7增殖和Rb基因表达的影响。〖HT5W〗方法： 〖HT5"SS〗采用RNAi技术对乳腺癌细胞MCF7细胞BRCAA1基因进行特异性抑制,用阳离子脂质体与化学合成的Predesigned antiBRCAA1 siRNA构建转染复合体,反转染MCF7细胞株48h后提取总RNA,分为未处理(NT)组、阴性对照组、阳性对照组和BRCAA1组,经反转录荧光实时PCR检测BRCAA1和Rb基因mRNA表达情况;检测细胞增殖抑制率。〖HT5W〗结果： 〖HT5"SS〗与阴性对照组相比,siRNA转染MCF7细胞后实验组BRCAA1基因mRNA水平降低了42.3％;Rb基因表达较之阴性对照组上升了11.1％;实验组MCF7细胞增殖抑制率为（81.9±6.1）％,抑制作用明显强于对照组(P<0.05)。〖HT5W〗结论： 〖HT5"SS〗BRCAA1基因的封闭明显抑制了MCF7细胞的增殖,BRCAA1基因与Rb基因可能存在有某种相互拮抗的作用。
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| 关 键 词： | BRCAA1 基因 乳腺癌 Rb基因 |
| 文章编号： | 1007-385X(2006)03-0181-04 |
| 收稿时间： | 2006-06-02 |
| 修稿时间： | 2006-06-12 |
Effect of blocking BRCAA1 gene with siRNA on proliferation of MCF-7 cells and expression of Rb gene |
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| YANG Hao,CUI Da-xiang,LI Qing,HUANG Tuo,GAO Feng,HE Rong,PAN Bi-feng,SHAO Jun,YOU Xiao-gang and LIU Feng-tao. Effect of blocking BRCAA1 gene with siRNA on proliferation of MCF-7 cells and expression of Rb gene[J]. Chinses Journal of Cancer Biotherapy, 2006, 13(3): 181-184 |
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| Authors: | YANG Hao CUI Da-xiang LI Qing HUANG Tuo GAO Feng HE Rong PAN Bi-feng SHAO Jun YOU Xiao-gang LIU Feng-tao |
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| Affiliation: | Department of Bio Nano Science and Engineering, Key Lab For Thin Film and Microfabrication of Ministry of Education, National Key Lab of Micro Nano Fabrication Technology, Institute of Micro Nano Science and Technology, Shanghai Jiaotong University, Shang;Department of Bio Nano Science and Engineering, Key Lab For Thin Film and Microfabrication of Ministry of Education, National Key Lab of Micro Nano Fabrication Technology, Institute of Micro Nano Science and Technology, Shanghai Jiaotong University, Shang;Department of Bio Nano Science and Engineering, Key Lab For Thin Film and Microfabrication of Ministry of Education, National Key Lab of Micro Nano Fabrication Technology, Institute of Micro Nano Science and Technology, Shanghai Jiaotong University, Shang;Department of Bio Nano Science and Engineering, Key Lab For Thin Film and Microfabrication of Ministry of Education, National Key Lab of Micro Nano Fabrication Technology, Institute of Micro Nano Science and Technology, Shanghai Jiaotong University, Shang;Department of Bio Nano Science and Engineering, Key Lab For Thin Film and Microfabrication of Ministry of Education, National Key Lab of Micro Nano Fabrication Technology, Institute of Micro Nano Science and Technology, Shanghai Jiaotong University, Shang;Department of Bio Nano Science and Engineering, Key Lab For Thin Film and Microfabrication of Ministry of Education, National Key Lab of Micro Nano Fabrication Technology, Institute of Micro Nano Science and Technology, Shanghai Jiaotong University, Shang;Department of Bio Nano Science and Engineering, Key Lab For Thin Film and Microfabrication of Ministry of Education, National Key Lab of Micro Nano Fabrication Technology, Institute of Micro Nano Science and Technology, Shanghai Jiaotong University, Shang;Department of Bio Nano Science and Engineering, Key Lab For Thin Film and Microfabrication of Ministry of Education, National Key Lab of Micro Nano Fabrication Technology, Institute of Micro Nano Science and Technology, Shanghai Jiaotong University, Shang;Department of Bio Nano Science and Engineering, Key Lab For Thin Film and Microfabrication of Ministry of Education, National Key Lab of Micro Nano Fabrication Technology, Institute of Micro Nano Science and Technology, Shanghai Jiaotong University, Shang;Department of Bio Nano Science and Engineering, Key Lab For Thin Film and Microfabrication of Ministry of Education, National Key Lab of Micro Nano Fabrication Technology, Institute of Micro Nano Science and Technology, Shanghai Jiaotong University, Shang |
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| Abstract: | |
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| Keywords: | siRNA |
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