| 沙棘果水溶多糖JS1的分离鉴定 |
| |
| 引用本文： | 王桂云,梁忠岩,张丽萍,苗春艳.沙棘果水溶多糖JS1的分离鉴定[J].中国药学杂志,1999,0(4):229-231. |
| |
| 作者姓名： | 王桂云 梁忠岩 张丽萍 苗春艳 |
| |
| 作者单位： | 长春130024东北师范大学生命科学学院 |
| |
| 摘 要： | 目的:从已提取过水溶性多糖后的沙棘果残渣中,进一步用稀碱液(0.1mol·L-1NaOH溶液)提取多糖JS1,并研究其组成性质。方法:利用DEAE-Sephadex A-25柱层析法纯化沙棘果碱提水溶多糖,并采用玻璃纤维纸电泳,Sepharos CL-4B柱层析比旋度等方法证明组分的均一性,同时用气相色谱、红外光谱等方法对其组成进行研究。结果:分离得到JS1水溶性多糖,经气相色谱分析得单糖组分为Ara,Xyl,Gal,Glc,摩尔比依次是1∶6∶12∶4?结论:JS1为中性杂多糖。
|
| 关 键 词： | 沙棘 沙棘多糖 分离 组成 |
| 收稿时间： | 1997-04-11; |
Purification and characterization of water soluble polysaccharide JS1 from Hippophae rhamnoides |
| |
| Wang Guiyun.Purification and characterization of water soluble polysaccharide JS1 from Hippophae rhamnoides[J].Chinese Pharmaceutical Journal,1999,0(4):229-231. |
| |
| Authors: | Wang Guiyun |
| |
| Institution: | (Wang GY),Liang Zhongyan(Hang ZY),Zhang Liping(Zhang LP),et a |
| |
| Abstract: | OBJECTIVE:To study the purification and characterization of water soluble polysaccharide JS1 from Hippophae rahamnoides L.fruits that had been extracted with water.METHODS:JS1 was purified by gel chromatography on DEAE-Sep-hadex A-25.its homogeneity was proved by gel chromatogrophy on Sepharose CL-4B glass fiber paper electrophoresis and specific rotator power.Its stral composition was studied by means of GC,IR,etc.RESULTS:JS1 is a water solube polysaccharide.It is consisted of Ara，Xyl，Gal，Glc with the molarity rate of 1∶6∶12∶4 in turn by means of GC.CONCLUSION:JS1 is a neutral heteropolysaccharide. |
| |
| Keywords: | Hippophae rhamnoides Hippophae rhamnoides polysaccharide isolation composition |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国药学杂志》浏览原始摘要信息 |
| 点击此处可从《中国药学杂志》下载免费的PDF全文 |
|
