| 脂肪组织扫描电镜样品制备方法 |
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| 引用本文： | Cui F,Liu C,Yang JM,Wang L. 脂肪组织扫描电镜样品制备方法[J]. 南方医科大学学报, 2012, 32(3): 435-436 |
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| 作者姓名： | Cui F Liu C Yang JM Wang L |
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| 作者单位： | 河北医科大学电镜实验中心;河北医科大学研究生学院;白求恩国际和平医院烧伤整形科 |
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| 摘 要： | 脂肪组织由于自身脂类含量丰富,采用常规扫描电镜生物样品制备法,很难达到预期效果。经过数次摸索,作者在常规制备样品基础上,增加了锇酸后固定,以使脂肪酸和磷脂蛋白得到稳定,即而稳定细胞膜结构;为使组织细胞内有机溶剂充分置换,又延长脱水时间。通过对这些具体操作细节进行改良,取得了较为满意的结果。
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| 关 键 词： | 扫描电镜 脂肪组织 样品制备 |
Adipose tissue sample preparation for scanning electron microscopy |
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| Cui Fang,Liu Chao,Yang Jian-Min,Wang Li. Adipose tissue sample preparation for scanning electron microscopy[J]. Journal of Southern Medical University, 2012, 32(3): 435-436 |
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| Authors: | Cui Fang Liu Chao Yang Jian-Min Wang Li |
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| Affiliation: | Laboratory Center of Electron Microscopy, Hebei Medical University, Shijiazhuang 050017, China. fanghua1980@hotmail.com |
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| Abstract: | Conventional sample preparation technique for adipose tissue is difficult to achieve satisfactory results for scanning electron microscopy (SEM). We adopted a strategy of postfix with osmium tetroxide to stabilize the fatty acids, phospholipids protein, and hence the membrane structure. Also by extending the dehydration time to fully replace the organic solvents, we achieved satisfactory results for SEM of adipose samples. |
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| Keywords: | scanning electron microscope adipose tissue sample preparation |
| 本文献已被 CNKI PubMed 等数据库收录！ |
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