| 乳糖诱导重组人甲状旁腺素（1-34）高密度发酵研究 |
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| 引用本文： | 杨立明,郑炎淮,李咏华,叶学君,阳勇,何凯,韩为跃.乳糖诱导重组人甲状旁腺素（1-34）高密度发酵研究[J].航空航天医药,2012,23(7):791-793. |
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| 作者姓名： | 杨立明 郑炎淮 李咏华 叶学君 阳勇 何凯 韩为跃 |
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| 作者单位： | 广东联康生物与医药研究院,广东,东莞,523581 |
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| 摘 要： | 利用乳糖诱导工程菌pET(32a+)-PTH(1-34)/BL21(DE3) 高效表达人甲状旁腺激素1-34融合蛋白.并对乳糖浓度、诱导温度、诱导时机等参数进行优化,得到目的蛋白高表达乳糖诱导高密度发酵工艺.三批实验结果表明,人甲状旁腺激素1-34融合蛋白表达量在33%左右,工程菌生物量在55 g/L左右.
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| 关 键 词： | 重组人甲状旁腺激素1-34 乳糖诱导 高密度发酵 |
Induced by Lactose for High-density Fermentation of Recombinant Human Parathyroid Hormone(1-34) |
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| Institution: | YANG Liming,ZHENG Yanhuai,LI Yonghua,et al.(Uni-Bio Institute of Biology ＆ Medicine Guangdong,Dongguan 523581,China) |
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| Abstract: | An engineering bacterial strain pET（32a＋）-PTH（1-34）/BL21（DE3） which can express the fusion protein of human parathyroid hormone（1-34） has been studied for high-density fermentation induced by lactose in this paper.Through the optimization of lactose concentration,the temperature of induction and the time of induction,we got a high-density fermentation process which can high performance express target protein induced by lactose.Three lots of engineering bacterial strain were cultured,and the results showed that the expression of human parathyroid hormone 1-34 fusion protein was 33% and the weight of bacteria strain was 55g/L approximately. |
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| Keywords: | Recombination of human parathyroid hormone 1-34 Lactose induction High-dendity fermentation |
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