银杏叶提取物对体外培养翼状胬肉成纤维细胞增殖的抑制作用

目的：研究不同浓度的银杏叶提取物（ginkgo biloba extract,GBE）对体外培养人翼状胬肉成纤维细胞(human pterygium fibroblasts，HPFs)增殖和凋亡的影响。 方法：用噻唑蓝比色法(MTT)检测不同浓度(12.5，25.0，50.0，100.0mg/L) GBE在不同作用时间(24，48，72h)对体外培养的HPFs增殖的影响。用流式细胞仪检测HPFs的细胞周期及凋亡率。 结果：MTT结果显示，GBE浓度为12.5mg/L组作用24h后对HPFs的增殖无明显抑制作用（P>0.05），其余各时间段各组均可见明显抑制HPFs的增殖，且各组间比较差异有统计学意义(P<0.05)；流式细胞仪检测发现GBE作用48h后G₀/G₁期细胞比例增加，S期细胞比例下降，计算不同浓度GBE作用下凋亡率分别为4.37%,8.14%,11.09%,15.18%,组间比较差异有统计学意义(P<0.05)。 结论：GBE对HPFs的增殖有明显抑制作用，并与用药剂量和持续时间有关。

Ginkgo biloba extract inhibits the proliferation of human pterygium fibroblasts in vitro

AIM:To investigate the effects of ginkgo biloba extract(GBE) with different concentration on the proliferation of human pterygium fibroblasts cells(HPFs).METHODS:The HPFs were incubated with GBE at concentration of 12.5mg/L,25.0mg/L,50.0mg/L,100.0mg/L for 24-72 hours,the effects of GBE on the proliferation of HPFs were evaluated by MTT assay.Flow cytometry(FCM) was used to examine the cell cycle distribution and apoptosis of HPFs exposed to different concentrations of GBE for 48 hours.RESULTS:The proliferation of HPFs was not significantly inhibited incubated with 12.5mg/L GBE for 24 hours through MTT assay(P>0.05).Other groups could significantly inhibit HPFs proliferation in a dose-and time-dependent manner,and there were significant differences between each of the two groups(P<0.05).Flow cytometry examination suggested the cell percentage in the G0/G1 phase increased while that of the S phase decreased exposed to different concentrations of GBE for 48 hours,the rate of apoptosis of HPFs respectively reached 4.37%,8.14%,11.09%,15.18%,and there were significant differences between the two groups(P<0.05).CONCLUSION:GBE can significantly inhibit HPFs proliferation and induce its apoptosis with dose-and time-dependent manner.