牛磺酸对兔RPE细胞光损伤的保护作用

目的：应用SCGE法检测牛磺酸对家兔视网膜色素上皮细胞可见光损伤的保护作用。方法：取2~4代的家兔色素上皮细胞建立光损伤模型，然后分别应用40mmol/L和80mmol/L浓度牛磺酸液对光损伤的细胞继续培养24h，并设立无牛磺酸培养的对照组。应用单细胞凝胶电泳法分别测定各组细胞DNA的改变。结果：光损伤后的家兔RPE细胞经过牛磺酸液培养后DNA损伤程度均有所减轻，尤其80mmol/L剂量组的保护作用更为明显，细胞的尾长、彗星细胞百分率与对照组相比均有显著差异(P<0.01)。结论：牛磺酸中的有效成分对家兔RPE光损伤有明显的保护作用，且呈剂量依赖的量效关系。

Protection effect of taurine on photic injury in RPE cells of rabbits

AIM:To detect the protection effect of taurine on photic injury in retinal pigment epithelial(RPE) cells of rabbits with single-cell gel electrophoresis assay(SCGE).METHODS:The 2-4 passage RPE cells of rabbits were adopted to establish visible high damage model.The cultured RPE cells were pretreated with taurine of 40mmol/L,80mmol/L for 24 hours,whereas control group was pretreated without taurine.The cell’s DNA damage of various groups was detected with SCGE.RESULTS:The DNA damage on RPE cells of rabbits was reduced,80mmol/L group had obvious effect than the other groups.There was significant difference with control group in tail DNA% and tail length(P<0.01).CONCLUSION:Taurine can protect RPE cells in rabbits against photic injury.Its effect is does-dependent.