超滤膜技术分离杜仲叶绿原酸的研究

目的：研究超滤膜技术应用于杜仲叶绿原酸的分离纯化。方法：以绿原酸转移率为指标,考察料液温度、超滤压力、超滤时间、膜截留分子量对绿原酸转移率的影响。结果：膜截留分子量200000,超滤温度40℃、超滤压力0.36MPa、超滤时间20min、加水量24L时,绿原酸转移率为99.38%。结论：超滤膜技术分离纯化杜仲叶绿原酸效果较好,可应用于产业化。

Study on Isolation of Chlorgenic Acid from Eucommia ulmoides Leaves by Ultrafiltration Technique

OBJECTIVE: To isolate and purify Chlorogenic acid from Eucommia ulmoides leaves by ultrafiltration technique. METHODS: Chlorogenic acid transferring ratio was investigated by temperature, pressure, time, cut-off molecular mass. RESULTS: The optimal conditions for the ultrafiltration technique were finally determined. It was found that, for the membrane with a cut-off molecular mass of 20000, when the temperature, pressure, time were respectively 40 degrees C, 0.36 MPa, 20 minutes and adding 24L water, yield of Chlorogenic acid was 99.38%. CONCLUSION: High Chlorogenic acid transfering ratio can be gotten by ultrafiltration technique, which is helpful to the further isolation and purification of Chlorogenic acid in the subsequent process.