活体成年小鼠舌黏膜下植入蛋白凝胶颗粒的方法学研究

目的：建立活体动物舌黏膜植入外源性蛋白凝胶颗粒的方法。方法：出生后90.5d的C57BL小鼠经戊巴比妥钠麻醉后,于舌黏膜下植入小牛血清（bovineserum albumin,BSA）的凝胶颗粒。实验后48h,取小鼠舌组织固定、脱水、包埋、切片后进行HE染色、Brdu染色及PCNA染色。结果：实验后48h,小鼠舌黏膜无明显炎性病变,染色结果显示凝胶颗粒的植入对上皮内BrdU和PCNA的表达无影响。结论：本研究成功地建立了舌黏膜下植入蛋白凝胶颗粒的方法,为将来药物和蛋白质诱导活体动物器官局部增殖、分化和凋亡奠定基础。

A mouse model of agarose beads implantation in the tongue

Objective： To establish a method in which exogenous agarose beads are implanted under the epithelium of the tongue in adult mouse. Methods： 10 male C57 BL mice of postnatal 90.5 days old were selected in this study. After anesthetized by injection of pentobarbital sodium, the agarose beads soaked with BSA were implanted under the epithelium of the tongues. 48 hours later, the mice were sacrificed, and the tongues were dissected, fixed in 4% paraformaldehyde,dehydrated with alcohol, embedded in wax and cut into slices for HE and immunohistochemical staining. Results： After 48 hours, all mice were alive, and there were no obvious inflammatory lesions on the tongue mucosa. The histological results showed that the beads were located submucously in the tongue and the implanted protein did not influence the expression of BrdU and PCNA in the lingual epithelium. Conclusion： we successfully established an animal model to implant agarose beads soaked with proteins under the mouse＇s epithelium in vivo.