SNAP25基因多态性对氨磺必利治疗精神分裂症的PANSS评分的影响

目的 研究突触相关蛋白25 kDa(synaptosomal associated protein ofS25KD,SNAP25)基因rs8636位点和rs363032位点单核苷酸多态性与精神分裂症易感性的相关性,并评估其对氨磺必利治疗精神分裂症患者PANSS评分的影响。方法 选择150例精神分裂症作为研究组,招募145例健康体检者作为对照组。采用Sanger测序法分析所有受试者SNAP25基因rs8636位点和rs363032位点基因型。结果 SNAP25基因rs8636位点T等位基因和rs363032位点C等位基因是精神分裂症的危险因素(校正OR=1.21,95%CI=1.02-1.43,P=0.03；校正OR=1.60,95%CI=1.35-1.83,P<0.001)。SNAP25基因rs8636位点和rs363032位点野生型、杂合型、突变纯合型患者治疗消耗的氨磺必利的剂量依次升高,差异有统计学意义(P<0.05)。治疗前与治疗后SNAP25基因rs8636位点和rs363032位点不同基因型之间的PANSS总分、阳性症状评分、阴性症状评分、一般病理症状评分之间的差异没有统计学意义与(P>0.05)。SNAP25基因rs8636位点和rs363032位点不同基因型精神分裂症患者的不良反应情况之间的差异均没有统计学意义(P>0.05)。结论 SNAP25基因rs8636位点和rs363032位点单核苷酸多态性与精神分裂症易感性有关,突变型精神分裂症患者需要更大剂量的氨磺必利进行治疗。

Effect of polymorphism of SNAP25 gene on PANSS score of amisulpride in the treatment of schizophrenia.

Objective To study the association between single nucleotide polymorphisms of rs8636 and rs363032 in the synapsosome associated protein 25 kD (SNAP25) gene and the susceptibility to schizophrenia, and to evaluate its Amisulpride in the treatment of schizophrenia patients with PANSS score. Methods One hundred and fifty patients with schizophrenia were selected as study groups and 145 healthy individuals were recruited as control groups. Sanger sequencing was used to analyze the genotypes of the rs8636 and rs363032 loci of the SNAP25 gene in all subjects. Results The T allele of rs8636 in the SNAP25 gene and the C allele in the rs363032 locus were risk factors for schizophrenia (adjusted OR=1.21, 95%CI=1.02-1.43, P=0.03; adjusted OR=1.60, 95%CI=1.35-1.83, P<0.001). The doses of amisulpride consumed in patients with wild-type, heterozygous, and homozygous mutations at rs8636 and rs363032 in SNAP25 were increased in turn and the difference were statistically significant (P<0.05). There was no significant difference between the PANSS total score, positive symptom score, negative symptom score and general pathological symptom scores of the SNAP25 gene rs8636 locus and rs363032 locus before and after treatment (P>0.05). There was no significant difference in adverse reactions between schizophrenia patients with different genotypes of rs8636 locus and rs363032 locus of SNAP25 (P>0.05). Conclusion The SNP25 gene rs8636 loci and rs363032 loci are associated with susceptibility to schizophrenia. Mutant schizophrenia patients require a higher dose of amisulpride for treatment.