应用基因芯片技术筛选生长激素腺瘤相关基因

目的应用基因芯片技术筛选人生长激素腺瘤发生、发展相关基因,为后续研究奠定基础。方法应用人全基因组寡核苷酸芯片HG-U133 Plus2.0检测8例生长激素腺瘤组织、2例混合正常垂体组织的基因表达谱,差异表达基因进行筛选和生物信息学分析。Real-time qPCR验证芯片结果。结果筛选出与生长激素腺瘤相关的上调基因187条,下调基因899条;差异表达基因功能主要涉及分子结合、凋亡或肿瘤相关、代谢、信号转导、细胞周期、物质运输等多个生物过程。结论基因芯片技术可初步筛选出人生长激素腺瘤相关基因;生长激素腺瘤的发生、发展是多基因、多分子、多通路的网络调控过程。

Whole genome oligonucleotide microarrays in screening of growth homone adenoma associated genes

Objective To screen for differentially expressed genes associated with the development and progression of human growth hormone adenoma,so as to lay a foundation for future study.Methods The whole genome oligonucleotide microarray(Affymetrix 133plus 2.0)was used to examine gene profiles of 8growth homone adenoma samples and 2normal pooled pituitary samples.Differentially expressed genes were subjected to bioinformatics analysis.Real-time qPCR was used to verify the microarray result of a randomly selected ca...