用生物发光法定量测定绿脓杆菌对聚苯乙烯的粘附

本文用生物发光技术建立了定量测定绿脓杆菌(PA)对聚苯乙烯表面粘附的研究方法,同时观察了影响 PA 粘附的某些因素,比较了12株 PA 菌株对聚苯乙烯的粘附率。结果表明:在生物发光(BL)反应体系中,在萤虫素/萤虫素酶浓度给定的条件下,发光强度与 PA 释放的 ATP 量呈剂量依赖关系;在 PA 粘附体系中,其与聚苯乙烯表面的粘附随菌量的增加和粘附时间的延长而增加并受其缓冲液 pH 和菌龄的影响;不同 PA 菌株在同一条件下对聚苯乙烯的粘附率具有很大差别。

Quantification of Adherence of Pseudomonas aeruginosa to Polystyrene by Using Bioluminescence Method

A Biouminescence method was established for quantification of adhesion of Pseudomonas aeruginosa to polystyrene,some factors influencing the method were observed and the ratio o0f P.aeruginosa strains were compared by the established method.The results showed: 1,In bioluminescence reaction,there was a dose-dependent relationship between the amonnt of ATP of P.aeruginosa and the intensity of bioluminescence in the given concentration of luciferin/luciferase;2, In the adherent system of P.aeruginosa,the amount of the adherence of P.aeruginosa to polystyrenc increased with the amount of the bacteria and prolongation of incubation time and was affected by pH of PBS,the age of P.aeruginosa;3,The adherence ratio of P.aeruginosa was significantly different among different P.aeruginosa strains.