| 高效液相色谱-示差折光检测法同时测定替硝唑葡萄糖注射液中替硝唑与葡萄糖的含量 |
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| 引用本文： | 周礼玲,张新芹. 高效液相色谱-示差折光检测法同时测定替硝唑葡萄糖注射液中替硝唑与葡萄糖的含量[J]. 数理医药学杂志, 2011, 24(6): 745-747. DOI: 10.3969/j.issn.1004-4337.2011.06.041 |
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| 作者姓名： | 周礼玲 张新芹 |
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| 作者单位： | 安徽省六安市食品药品检验所,六安,237006 |
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| 摘 要： | 目的:建立高效液相色谱-示差折光检测法测定替硝唑葡萄糖注射液含量的方法。方法:采用示差折光检测器,色谱柱采用氨基键合硅胶色谱柱(416mm×150mm,5μm;流动相为乙腈0.02mol.L-1 KH2P3O4溶液(pH6.0)70:30;柱温30℃,流速为1.0ml/min。结果:替硝唑和葡萄糖分别在0.10～0.50和1.25～6.25g.L-1的浓度范围内有良好的线性关系r=0.9998;r=0.9999,两者的平均回收率分别为100.3%与99.8%,RSD分别为1.1%与1.0%。结论:该法简便、快速、准确,适用于替硝唑葡萄糖注射液的含量测定。
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| 关 键 词： | 替硝唑 葡萄糖 含量测定 高效液相色谱 示差折光检测法 |
Simultaneous Assay of Tinidazole and Glucose in Tinidazole and Glucose Injection by HPLC with RID |
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| Zhou Liling,et al. Simultaneous Assay of Tinidazole and Glucose in Tinidazole and Glucose Injection by HPLC with RID[J]. Journal of Mathematical Medicine, 2011, 24(6): 745-747. DOI: 10.3969/j.issn.1004-4337.2011.06.041 |
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| Authors: | Zhou Liling et al |
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| Affiliation: | Zhou Liling,et al(Liuan Institute for Food and Drug Control,Liuan 237006) |
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| Abstract: | Objective： To establish a method using HPLC-RID for simultaneous assay of Tinidazole and Glucose in Tinidazole and Glucose Injection.Methods： The detector was differential refraction detector.The Kromasil 100-5NH2（416 mm ×150 mm,5μm） set at 30℃ was used.The mobile phase was composed of acetonitrile ：0.02 mol·L-1 KH2 PO4 solution（70：30）（pH 6.0）.Results： The linear range of Tinidazole was 0.10～0.50（r=0.9998）,and Glucose was1.25～6.25g·L-1（r=0.9999）.The average recovery of Tinidazole was 100.3%（RSD 1.1%）,and the average recovery of Glucose was 99.8%（RSD 1.0 %）. Conclusions： This method is efficient,accurate and suitable for the simultaneous assay of Tinidazole and Glucose in Tinidazole and Glucose Injection. |
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| Keywords: | tinidazole glucose Assay HPLC RID |
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