左旋精氨酸对大鼠失血性休克再灌注心脏的保护作用

目的探讨失血性休克再灌注心脏损伤变化及左旋精氨酸的保护作用。方法健康SD大鼠30只,随机均分成正常对照组(NC组)、失血性休克再灌注组(HS组)和左旋精氨酸治疗组(L-Arg组)。实验结束时,取血进行心功能检测,并测定超氧化物歧化酶(SOD)和黄嘌呤氧化酶(XOD)活性。结果与NC组相比较,HS组和L-Arg组血浆CK含量均明显升高,HS组心脏组织SOD活性显著下降,XO活性明显增高,而L-Arg组心脏组织的SOD活性无显著下降,XO活性亦无显著升高;L-Arg组血浆CK含量和XO活性明显低于HS组,而SOD活性明显高于HS组。结论大鼠失血性休克复苏后,心功能下降,氧自由基参与心脏的损伤过程,L-Arg通过抑制氧自由基的产生,减轻脂质过氧化反应发挥对心脏的保护作用。

The Protective Effects of L - arginine on Heart Resuscitation Injury After Hemorrhagic Shock in Rats

Objective To observe the protective effect of L-arginine on heart resuscitation injury after hemorrhagic shock in rats and ex- plore the mechanism of injury in hemorrhagic shock resuscitation rats.Methods 30 SD rats were randomly divided into 3 group:normal control (NC)group(n=10),hemorrhagic shock resuscitation(HS)group(n=10)and L-arginine(L-Arg)group(n=10).The CK content in plasm and the SOD,XO in heart tissue were detected.Results In HS group and L-Arg group,plasm CK content increased remarkably com- pared to NC group;In HS group,the SOD activity in heart tissue reduced and XO activity increased remarkably compared with the NC group.However,in L-Arg group,the SOD activity in heart tissue has not reduced and XO activity increased with fraee compared with the NC group.In L-Arg group,plasm CK content and XO activity were markedly lower than daose in HS group,while SOD and activity were signifi- candy higher than that in HS group.Conclusion:The heart function decreases after resuscitation in hemorrhagic shock of rats;the oxygen free radicals can cause heart injury;L-Arginine has protective effects on HS heart injury because of blocking the generation of OFR,elevating the ability of antioxidization and its anti-lipid peroxidation reaction.