人眼小梁网细胞的体外培养

目的 探讨体外培养人眼小梁网细胞的方法并观测其生物学特性。方法 采用组织块培养方法进行体外细胞培养，二甲基噻唑二苯基四唑溴盐比色法、倒置显微镜、透射电镜，免疫细胞化学等方法观察体外培养细胞的生物学特性并进行鉴定。结果 组织块培养7～15d后可见细胞从其边缘向外生长。倒置显微镜下成梭形、圆形、椭圆形、并有多个突起；电镜可见小梁网细胞表面有较多微绒毛，细胞间缝隙连接，胞质内见很多次级溶酶体。免疫细胞化学检测纤维黏连蛋白、层黏连蛋白和神经元特异性烯醇化酶染色阳性。这些特征可与角膜内皮细胞和巩膜成纤维细胞相鉴别。结论 组织培养方法能够成功体外培养人眼小梁网细胞。

Culture of Human Trabecular Meshwork Cells in Vitro

Objective To culture of human trabecular meshwork cells in vitro and for investigate their biological character. Methods The trabecular tissues from human eyes were cultured. The morphologic features and growing characteristics of the cultured cells were observed by inverted microscope, electronic microscope and immunohistochemistry. The proliferative curve of the cultured cells was obtained by colorimetric assay with MTT. Results The primarily cells in culture are observed from the edge of the cultured tissues after 10-15 days. The cultured cells were round, oval, fusiform and multiangular. Trabecular cells showed apical villons projections and had a high density of secondary lysosome. The junctions between trabecular cells observed frequently as a gap junction. Laminin(LN), fibronectin(FN) and neuron specific enolase(NSE) of the cultured cells were identified by immunohistochemical method. Conclusion Human trabecular meshwork cells can be successfully cultured in vitro.