促红细胞生成素对全脑缺血再灌注损伤大鼠血红素加氧酶-1表达的影响

目的探讨促红细胞生成素（EPO）对大鼠脑缺血再灌注后血红素加氧酶1（HO-1）表达的影响。方法随机将雄性SD大鼠分为缺血再灌注组、治疗组、假手术组。治疗组按照缺血再灌注后不同时间点给予EPO腹腔注射，假手术组不手术，于各时间点断头取脑，用逆转录-聚合酶链反应技术检测大鼠皮层HO-1 mRNA的表达。结果① 全脑缺血再灌注后HO-1 mRNA的表达发生动态变化，缺血后6h开始明显升高，24h达高峰，此后缓慢下降；② EPO能够显著上调全脑缺血后皮层HO-1 mRNA的表达,缺血再灌注后即注射EPO组与缺血再灌注组各时间点HO-1和β-actin吸光度的比值差异有统计学意义（P＜0.05 ）。结论EPO的神经保护作用可能部分通过上调HO-1的表达来实现。

Effects of erythropoietin on heme oxygenase-1 expression in rats with globle ischemia-reperfusion injury

To explore the effect of erythropoietin（EPO）treatment on the expression of heme oxygenase-1（HO-1）mRNA in rats with globe ischemia-reperfusion injury. Methods The 4-vessel obstructive globe ischemic rat models were used in this study. The rats were randomly divided into three groups: the ischemia-reperfusion group, the experimental group and sham-operation group. Rats in the ischemia reperfusion group and the experimental group were subjected to EPO injection into the abdominal cavity after globe ischemia, however rats in the sham-operation group were not subjected to an operation. The expression of HO-1 mRNA was determined by RT-PCR. Results① After ischemia, the expression of HO 1 mRMA was obviously changed based on time. ② The effect of EPO treatment on the expression of HO-1 mRNA in the cortex of this rat model was obvious. ConclusionThe protective effect of EPO for ischemia reperfusion injury is perhaps induced by up-regulating the expression of HO-1.