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| 鹿角托盘水溶性蛋白的提取分离与鉴定 | |
| 引用本文： | 黄萍,赵雨,牛放,唐仁能,李银清,刘诗超,张连学.鹿角托盘水溶性蛋白的提取分离与鉴定[J].亚太传统医药,2010,6(1):28-30. |
| 作者姓名： | 黄萍 赵雨 牛放 唐仁能 李银清 刘诗超 张连学 |
| 作者单位： | 1. 吉林农业大学中药材学院,吉林长春,130118 2. 长春中医药大学中医药与生物工程研发中,心,吉林长春130117 3. 延边大学药学院,吉林延吉,133000 |
| 摘 要： | 目的:对鹿角托盘中的水溶性蛋白进行提取分离和鉴定。方法:采用中性缓冲液浸提和中空纤维膜过滤技术提取鹿角托盘水溶性总蛋白,经采用SDS-PAGE电泳、BCA蛋白浓度测定法,基质辅助激光解析/电离飞行时间质谱技术及数据库(NCBI)检索进行蛋白分析鉴定。结果:鹿角托盘水溶性总蛋白的收率为8%,蛋白纯度为86%。在水溶性总蛋白中鉴定出5种蛋白,分别为:β-3亚型血红蛋白、抗菌肽1、肽聚糖识别蛋白、β-c亚型血红蛋白、Pre-pro血清白蛋白。结论:鹿角托盘中的水溶性蛋白与药材临床疗效密切相关。 |
| 关 键 词： | 鹿角托盘 水溶性蛋白 提取和分离 鉴定 |
Isolation and Identification of Sika Antlerbase Soluble Proteins |
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| Hang Ping,Zhao Yu,Niu Fang,Tang Renneng,Li Yingqing,Liu Shichao,Zhang Lianxue.Isolation and Identification of Sika Antlerbase Soluble Proteins[J].Asia-Pacific Traditional Medicine,2010,6(1):28-30. | |
| Authors: | Hang Ping Zhao Yu Niu Fang Tang Renneng Li Yingqing Liu Shichao Zhang Lianxue |
| Institution: | 1.College of Traditional Chinese Medicine Materials,Jilin Agricultural University, Changchun 130118, China ;2.Center for New Medicine Research of Changchun University of Traditional Chinese Medicine, Changchun 130117, China; 3.College of Pharmacy, Yanbian University, Yanji 133000, China) |
| Abstract: | Objective：To extract,isolate and identify soluble proteins of Sika antlerbase.Methods：Using Neutral buffer extraction and hollow fiber membrane filtration technology to extract soluble total proteins of Sika antlerbase. To analyze and identify the proteins by SDS-PAGE electrophoresis,BCA assay concentration, matrix-assisted laser desorption / ionization time-of-flight mass spectrometry and database （NCBI） searches .Results：The yield is 8% for soluble total protein of Sika antlerbase, the purification is 86%. We has identified five proteins, respectively： Hemoglobin subunitβ-3; Cathelicidin 1; Peptidoglycan recognition protein; Hemoglobin subunitβ-c ; Pre-pro serum albumin.Conclusion：soluble Proteins of Sika antlerbase is closely linked with its clinical efficacy. |
| Keywords: | Sika antlerbase Soluble protein Extraction and Separation Identification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |