| Fabrication of oligonucleotide microarray for the detection of Japanese encephalitis virus |
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| 引用本文： | HAI YAN ZHANG,WEN LI MA,XIAO MING ZHANG and WEN LING ZHENG Institute of Molecular Biology,Southern Medical University,Guangzhou,P.R. China Center of Disease Control of Guangzhou Military District,Guangzhou,P.R. China Liu Hua Qiao Hospital,Guangzhou,P. R. China. Fabrication of oligonucleotide microarray for the detection of Japanese encephalitis virus[J]. 中华微生物学和免疫学杂志(英文版), 2006, 4(2): 96-99 |
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| 作者姓名： | HAI YAN ZHANG WEN LI MA XIAO MING ZHANG and WEN LING ZHENG Institute of Molecular Biology Southern Medical University Guangzhou P.R. China Center of Disease Control of Guangzhou Military District Guangzhou P.R. China Liu Hua Qiao Hospital Guangzhou P. R. China |
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| 作者单位： | HAI YAN ZHANG，WEN LI MA，XIAO MING ZHANG and WEN LING ZHENG Institute of Molecular Biology，Southern Medical University，Guangzhou，P.R. China Center of Disease Control of Guangzhou Military District，Guangzhou，P.R. China Liu Hua Qiao Hospital，Guangzhou，P. R. China |
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| 摘 要： | A low-density oligonucleotide microarray was used for the detection of Japanese encephalitis virus (JEV) , combining with restriction display PCR labeling method. The hybridization targets were amplified from 6 plasmids containing several JEV gene fragments. Corresponding oligonucleotide probe spots were detected unambiguously. We claim that the oligonucleotide microarray technology is feasible and may have potential for clinical laboratory application.
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| 关 键 词： | 寡核苷酸 脑炎 病毒 杂化作用 生物化学 |
Fabrication of oligonucleotide microarray for the detection of Japanese encephalitis virus |
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| HAI YAN ZHANG WEN LI MA XIAO MING ZHANG WEN LING ZHENG. Fabrication of oligonucleotide microarray for the detection of Japanese encephalitis virus[J]. Chinese Journal of Microbiology and Immunology, 2006, 4(2): 96-99 |
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| Authors: | HAI YAN ZHANG WEN LI MA XIAO MING ZHANG WEN LING ZHENG |
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| Affiliation: | [1]Institute of Molecular Biology, Southern Medical University, Guangzhou, P. R. China [2]Center of Disease Control of Guangzhou Military District, Guangzhou, P. R. China [3]Liu Hua Qiao Hospital, Guangzhou, P. R. China |
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| Abstract: | A low-density oligonucleotide microarray was used for the detection of Japanese encephalitis virus (JEV) , combining with restriction display PCR labeling method. The hybridization targets were amplified from 6 plasmids containing several JEV gene fragments. Corresponding oligonucleotide probe spots were detected unambiguously. We claim that the oligonucleotide microarray technology is feasible and may have potential for clinical laboratory application. |
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| Keywords: | Japanese encephalitis virus Labeling Microarray Oligonucleotide Restriction display PCR |
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